Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1

David Kolodrubetz; Wendy Haggren; Alex Burgum

doi:10.1016/0014-5793(88)80251-5

Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1

David Kolodrubetz, Wendy Haggren, Alex Burgum

Department of Microbiology, Immunology & Molecular Genetics

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.

Original language	English (US)
Pages (from-to)	175-179
Number of pages	5
Journal	FEBS Letters
Volume	238
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(88)80251-5
State	Published - Sep 26 1988

Keywords

(Saccharomyces cerevisiae)
Amino acid sequence
Antibody
Chromatin
High mobility group protein

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(88)80251-5

Cite this

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title = "Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1",

abstract = "Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.",

keywords = "(Saccharomyces cerevisiae), Amino acid sequence, Antibody, Chromatin, High mobility group protein",

author = "David Kolodrubetz and Wendy Haggren and Alex Burgum",

note = "Funding Information: AcknowledgementTsh:i s work was supported in part by a grant from the National Institutes of Health (GM34486) and by grant AQ-984 from the Robert A. Welch Foundation.",

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doi = "10.1016/0014-5793(88)80251-5",

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T1 - Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1

AU - Kolodrubetz, David

AU - Haggren, Wendy

AU - Burgum, Alex

N1 - Funding Information: AcknowledgementTsh:i s work was supported in part by a grant from the National Institutes of Health (GM34486) and by grant AQ-984 from the Robert A. Welch Foundation.

PY - 1988/9/26

Y1 - 1988/9/26

N2 - Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.

AB - Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.

KW - (Saccharomyces cerevisiae)

KW - Amino acid sequence

KW - Antibody

KW - Chromatin

KW - High mobility group protein

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JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

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Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1

Abstract

Keywords

ASJC Scopus subject areas

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