Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1

David J Kolodrubetz, Wendy Haggren, Alex Burgum

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.

Original languageEnglish (US)
Pages (from-to)175-179
Number of pages5
JournalFEBS Letters
Volume238
Issue number1
DOIs
StatePublished - Sep 26 1988

Fingerprint

HMGB1 Protein
Saccharomyces cerevisiae Proteins
Nuclear Proteins
Yeast
Chromatin
Proteins
Amino Acids
High Mobility Group Proteins
Thymus
Subcellular Fractions
Molecular mass
Eukaryotic Cells
Thymus Gland
Saccharomyces cerevisiae
Immune Sera
Amino Acid Sequence
Yeasts
Chemical analysis

Keywords

  • (Saccharomyces cerevisiae)
  • Amino acid sequence
  • Antibody
  • Chromatin
  • High mobility group protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1. / Kolodrubetz, David J; Haggren, Wendy; Burgum, Alex.

In: FEBS Letters, Vol. 238, No. 1, 26.09.1988, p. 175-179.

Research output: Contribution to journalArticle

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