Abstract
Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.
Original language | English (US) |
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Pages (from-to) | 175-179 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 238 |
Issue number | 1 |
DOIs | |
State | Published - Sep 26 1988 |
Keywords
- (Saccharomyces cerevisiae)
- Amino acid sequence
- Antibody
- Chromatin
- High mobility group protein
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology