Abstract
Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 175-179 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 238 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 26 1988 |
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Keywords
- (Saccharomyces cerevisiae)
- Amino acid sequence
- Antibody
- Chromatin
- High mobility group protein
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1. / Kolodrubetz, David J; Haggren, Wendy; Burgum, Alex.
In: FEBS Letters, Vol. 238, No. 1, 26.09.1988, p. 175-179.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Amino-terminal sequence of a Saccharomyces cerevisiae nuclear protein, NHP6, shows significant identity to bovine HMG1
AU - Kolodrubetz, David J
AU - Haggren, Wendy
AU - Burgum, Alex
PY - 1988/9/26
Y1 - 1988/9/26
N2 - Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.
AB - Several nonhistone chromatin proteins (NHPs) have been isolated from Saccharomyces cerevisiae nuclei. They have molecular masses and amino acid compositions typical of the high mobility group (HMG) proteins from higher eukaryotic cells. Polyclonal antisera raised against two of the NHPs have been used in immunoblots of proteins from subcellular fractions of yeast to show that the NHPs are indeed nuclear. In addition, the amino-terminal amino acid sequences of several of the NHPs were determined. Importantly, the amino-terminal sequence of one of the proteins, NHP6, has significant (60%) identity with a stretch of amino acids in calf thymus HMG1.
KW - (Saccharomyces cerevisiae)
KW - Amino acid sequence
KW - Antibody
KW - Chromatin
KW - High mobility group protein
UR - http://www.scopus.com/inward/record.url?scp=0023741397&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023741397&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(88)80251-5
DO - 10.1016/0014-5793(88)80251-5
M3 - Article
C2 - 3169249
AN - SCOPUS:0023741397
VL - 238
SP - 175
EP - 179
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -