TY - JOUR
T1 - Alternative variants of human HYDIN are novel cancer-associated antigens recognized by adaptive immunity
AU - Laske, Karoline
AU - Shebzukhov, Yuriy V.
AU - Grosse-Hovest, Ludger
AU - Kuprash, Dmitry V.
AU - Khlgatian, Svetlana V.
AU - Koroleva, Ekaterina P.
AU - Sazykin, Alexey Y.
AU - Penkov, Dmitry N.
AU - Belousov, Pavel V.
AU - Stevanovic, Stefan
AU - Vass, Verona
AU - Walter, Steffen
AU - Eisel, David
AU - Schmid-Horch, Barbara D.
AU - Nedospasov, Sergei A.
AU - Rammensee, Hans Georg
AU - Gouttefangeas, Cécile
N1 - Publisher Copyright:
©2013 AACR.
PY - 2013/9/1
Y1 - 2013/9/1
N2 - A mutation in the hydin gene has been recently described as one possible mechanism leading to lethal congenital hydrocephalus in mice, and a similar defect is proposed to be involved in an autosomal recessive form of hydrocephalus in human. Here, we report for the first time on the cancer association and immunogenicity of two HYDIN variants in humans. One is a previously described sequence derived from the chromosome 1 gene copy, that is, KIAA1864. The second is encoded by a novel alternative transcript originating from the chromosome 16, which we identified by immunoscreening of a testis-derived cDNA expression library with sera of patients with colorectal cancer, and called MO-TES391. Both variants are targeted by immunoglobulin G antibodies in a significant subset of cancer patients but only rarely in healthy donors. Moreover, we identify HLA-A*0201-restricted sequences derived from MO-TES391 and KIAA1864, which are specifically recognized by human cytotoxic CD8(+) T cells. Taken together, these results suggest frequent and coordinated adaptive immune responses against HYDIN variants in patients with cancer and propose HYDIN as a novel cancer-associated antigen.
AB - A mutation in the hydin gene has been recently described as one possible mechanism leading to lethal congenital hydrocephalus in mice, and a similar defect is proposed to be involved in an autosomal recessive form of hydrocephalus in human. Here, we report for the first time on the cancer association and immunogenicity of two HYDIN variants in humans. One is a previously described sequence derived from the chromosome 1 gene copy, that is, KIAA1864. The second is encoded by a novel alternative transcript originating from the chromosome 16, which we identified by immunoscreening of a testis-derived cDNA expression library with sera of patients with colorectal cancer, and called MO-TES391. Both variants are targeted by immunoglobulin G antibodies in a significant subset of cancer patients but only rarely in healthy donors. Moreover, we identify HLA-A*0201-restricted sequences derived from MO-TES391 and KIAA1864, which are specifically recognized by human cytotoxic CD8(+) T cells. Taken together, these results suggest frequent and coordinated adaptive immune responses against HYDIN variants in patients with cancer and propose HYDIN as a novel cancer-associated antigen.
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U2 - 10.1158/2326-6066.CIR-13-0079
DO - 10.1158/2326-6066.CIR-13-0079
M3 - Article
C2 - 24777681
AN - SCOPUS:84937511109
SN - 2326-6066
VL - 1
SP - 190
EP - 200
JO - Cancer Immunology Research
JF - Cancer Immunology Research
IS - 3
ER -