Altered macrophage phenotype transition impairs skeletal muscle regeneration

Hanzhou Wang, David W. Melton, Laurel Porter, Zaheer U. Sarwar, Linda M Mcmanus, Paula K Shireman

Research output: Contribution to journalArticle

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Abstract

Monocyte/macrophage polarization in skeletal muscle regeneration is ill defined. We used CD11b-diphtheria toxin receptor transgenic mice to transiently deplete monocytes/macrophages at multiple stages before and after muscle injury induced by cardiotoxin. Fat accumulation within regenerated muscle was maximal when ablation occurred at the same time as cardiotoxin-induced injury. Early ablation (day 1 after cardiotoxin) resulted in the smallest regenerated myofiber size together with increased residual necrotic myofibers and fat accumulation. However, muscle regeneration after late (day 4) ablation was similar to controls. Levels of inflammatory cells in injured muscle following early ablation and associated with impaired muscle regeneration were determined by flow cytometry. Delayed, but exaggerated, monocyte [CD11b+(CD90/B220/ CD49b/NK1.1/Ly6G)-(F4/80/I-Ab/CD11c)-Ly6C+/-] accumulation occurred; interestingly, Ly6C+ and Ly6C- monocytes were present concurrently in ablated animals and control mice. In addition to monocytes, proinflammatory, Ly6C+ macrophage accumulation following early ablation was delayed compared to controls. In both groups, CD11b+F4/80+ cells exhibited minimal expression of the M2 markers CD206 and CD301. Nevertheless, early ablation delayed and decreased the transient accumulation of CD11b+F4/80+Ly6C -CD301- macrophages; in control animals, the later tissue accumulation of these cells appeared to correspond to that of anti-inflammatory macrophages, determined by cytokine production and arginase activity. In summary, impairments in muscle regeneration were associated with exaggerated monocyte recruitment and reduced Ly6C- macrophages; the switch of macrophage/monocyte subsets is critical to muscle regeneration.

Original languageEnglish (US)
Pages (from-to)1167-1184
Number of pages18
JournalAmerican Journal of Pathology
Volume184
Issue number4
DOIs
StatePublished - 2014

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Regeneration
Monocytes
Skeletal Muscle
Macrophages
Phenotype
Cardiotoxins
Muscles
Fats
Arginase
Wounds and Injuries
Transgenic Mice
Flow Cytometry
Anti-Inflammatory Agents
Cytokines

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Altered macrophage phenotype transition impairs skeletal muscle regeneration. / Wang, Hanzhou; Melton, David W.; Porter, Laurel; Sarwar, Zaheer U.; Mcmanus, Linda M; Shireman, Paula K.

In: American Journal of Pathology, Vol. 184, No. 4, 2014, p. 1167-1184.

Research output: Contribution to journalArticle

Wang, Hanzhou ; Melton, David W. ; Porter, Laurel ; Sarwar, Zaheer U. ; Mcmanus, Linda M ; Shireman, Paula K. / Altered macrophage phenotype transition impairs skeletal muscle regeneration. In: American Journal of Pathology. 2014 ; Vol. 184, No. 4. pp. 1167-1184.
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