Alterations of innate immunity reactants in transition dairy cows before clinical signs of lameness

Guanshi Zhang, Dagnachew Hailemariam, Elda Dervishi, Qilan Deng, Seyed A. Goldansaz, Suzanna M. Dunn, Burim N. Ametaj

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The objectives of this study were to evaluate metabolic and innate immunity alterations in the blood of transition dairy cows before, during, and after diagnosis of lameness during periparturient period. Blood samples were collected from the coccygeal vain once per week before morning feeding from 100 multiparous Holstein dairy cows during –8, –4, disease diagnosis, and +4 weeks (wks) relative to parturition. Six healthy cows (CON) and six cows that showed clinical signs of lameness were selected for intensive serum analyses. Concentrations of interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor (TNF), haptoglobin (Hp), serum amyloid A (SAA), lipopolysaccharide binding protein (LBP), lactate, non-esterified fatty acids (NEFA), and β-hydroxybutyrate (BHBA) were measured in serum by ELISA or colorimetric methods. Health status, DMI, rectal temperature, milk yield, and milk composition also were monitored for each cow during the whole experimental period. Results showed that cows affected by lameness had greater concentrations of lactate, IL-6, and SAA in the serum vs. CON cows. Concentrations of TNF tended to be greater in cows with lameness compared with CON. In addition, there was a health status (Hs) by time (week) interaction for IL-1, TNF, and Hp in lameness cows vs. CON ones. Enhanced serum concentrations of lactate, IL-6, and SAA at –8 and –4 wks before parturition were different in cows with lameness as compared with those of the CON group. The disease was also associated with lowered overall milk production and DMI as well as milk fat and fat-to-protein ratio. In conclusion, cows affected postpartum by lameness had alterations in several serum variables related to innate immunity and carbohydrate metabolism that give insights into the etiopathogenesis of the disease and might serve to monitor health status of transition dairy cows in the near future.

Original languageEnglish (US)
Pages (from-to)717-747
Number of pages31
JournalAnimals
Volume5
Issue number3
DOIs
StatePublished - Aug 14 2015

Keywords

  • Acute phase proteins
  • Blood
  • Cytokines
  • Dairy cow
  • Lameness
  • Metabolites

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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