Allogeneic transplantation of normal epidermal cells and squamous cell carcinomas in SENCAR mice

Claudio J. Conti, Jo Ann W. Fries, Claudio M. Aldaz, Andres J.P. Klein-Szanto, Thomas J. Slaga

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A method used in our laboratory for the transplantation of single cell suspensions of epidermis and skin carcinomas is described. Silicone chambers were placed into a subcutaneous granulation tissue formed by the implantation of a glass disk. The skin was closed on top of the chamber by using Michael autoclips. Cells were injected 24 to 48 hr later through the skin using a syringe and needle. The neoformation of epithelia with adnexa was observed when newborn epidermal cells were injected. When a fresh cell suspension of squamous cell carcinoma was used, a typical differentiated carcinoma was formed within 2 to 4 weeks. However, after 4 weeks, some of the grafts showed mild signs of rejection. The technique described is a useful system to transplant squamous cell carcinomas and can also be used as a rapid assay for malignancy.

Original languageEnglish (US)
Pages (from-to)125-129
Number of pages5
JournalEnvironmental health perspectives
VolumeVol. 68
DOIs
StatePublished - 1986
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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    Conti, C. J., Fries, J. A. W., Aldaz, C. M., Klein-Szanto, A. J. P., & Slaga, T. J. (1986). Allogeneic transplantation of normal epidermal cells and squamous cell carcinomas in SENCAR mice. Environmental health perspectives, Vol. 68, 125-129. https://doi.org/10.1289/ehp.8668125