AKAP95-mediated nuclear anchoring of PKA mediates cortisol-induced PTGS2 expression in human amnion fibroblasts

Jiangwen Lu, Wangsheng Wang, Yabing Mi, Chuyue Zhang, Hao Ying, Luyao Wang, Yawei Wang, Leslie Myatt, Kang Sun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Phosphorylation of the transcription factors cyclic adenosine monophosphate response element-binding protein (CREB) and signal transducer and activator of transcription 3 (STAT3) by protein kinase A (PKA) is required for the cortisol-induced production of cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) in human amnion fibroblasts, which critically mediates human parturition (labor). We found that PKA was confined in the nucleus by A-kinase-anchoring protein 95 (AKAP95) in amnion fibroblasts and that this localization was key to the cortisolinduced expression of PTGS2, the gene encoding COX-2. Cortisol increased the abundance of nuclear PKA by stimulating the expression of the gene encoding AKAP95. Knockdown of AKAP95 not only reduced the amounts of nuclear PKA and phosphorylated CREB but also attenuated the induction of PTGS2 expression in primary human amnion fibroblasts treated with cortisol, whereas the phosphorylation of STAT3 in response to cortisol was not affected. The abundances of AKAP95, phosphorylated CREB, and COX-2 were markedly increased in human amnion tissue after labor compared to those in amnion tissues from cesarean sections without labor. These results highlight an essential role for PKA that is anchored in the nucleus by AKAP95 in the phosphorylation of CREB and the consequent induction of COX-2 expression by cortisol in amnion fibroblasts, which may be important in human parturition.

Original languageEnglish (US)
Article numbereaac6160
JournalScience Signaling
Volume10
Issue number506
DOIs
StatePublished - Nov 21 2017
Externally publishedYes

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Amnion
Cyclooxygenase 2
Fibroblasts
Nuclear Proteins
Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Hydrocortisone
Phosphotransferases
Phosphorylation
STAT3 Transcription Factor
Proteins
Gene encoding
Personnel
STAT Transcription Factors
Parturition
CREB-Binding Protein
Tissue
Gene Expression
Response Elements
Dinoprostone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

AKAP95-mediated nuclear anchoring of PKA mediates cortisol-induced PTGS2 expression in human amnion fibroblasts. / Lu, Jiangwen; Wang, Wangsheng; Mi, Yabing; Zhang, Chuyue; Ying, Hao; Wang, Luyao; Wang, Yawei; Myatt, Leslie; Sun, Kang.

In: Science Signaling, Vol. 10, No. 506, eaac6160, 21.11.2017.

Research output: Contribution to journalArticle

Lu, J, Wang, W, Mi, Y, Zhang, C, Ying, H, Wang, L, Wang, Y, Myatt, L & Sun, K 2017, 'AKAP95-mediated nuclear anchoring of PKA mediates cortisol-induced PTGS2 expression in human amnion fibroblasts', Science Signaling, vol. 10, no. 506, eaac6160. https://doi.org/10.1126/scisignal.aac6160
Lu, Jiangwen ; Wang, Wangsheng ; Mi, Yabing ; Zhang, Chuyue ; Ying, Hao ; Wang, Luyao ; Wang, Yawei ; Myatt, Leslie ; Sun, Kang. / AKAP95-mediated nuclear anchoring of PKA mediates cortisol-induced PTGS2 expression in human amnion fibroblasts. In: Science Signaling. 2017 ; Vol. 10, No. 506.
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