Airway Mucin 2 is decreased in patients with severe chronic obstructive pulmonary disease with bacterial colonization

Oriol Sibila, Laia Garcia-Bellmunt, Jordi Giner, Ana Rodrigo-Troyano, Guillermo Suarez-Cuartin, Alfons Torrego, Diego Castillo, Ingrid Solanes, Eder F. Mateus, Silvia Vidal, Ferran Sanchez-Reus, Ernest Sala, Borja G. Cosio, Marcos Restrepo, Antonio R Anzueto, James D. Chalmers, Vicente Plaza

Research output: Contribution to journalArticle

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Abstract

Rationale: Mucins are essential for airway defense against bacteria. We hypothesized that abnormal secreted airway mucin levels would be associated with bacterial colonization in patients with severe chronic obstructive pulmonary disease (COPD) Objectives: To investigate the relationship between mucin levels and the presence of potentially pathogenic micro-organisms in the airways of stable patients with severe COPD Methods: Clinically stable patients with severe COPD were examined prospectively. All patients underwent a computerized tomography scan, lung function tests, induced sputum collection, and bronchoscopy with bronchoalveolar lavage (BAL) and protected specimen brush. Patients with bronchiectasis were excluded. Secreted mucins (MUC2, MUC5AC, and MUC5B) and inflammatory markers were assessed in BAL and sputum by ELISA. Measurements and Main Results:Weenrolled 45 patients, with mean age (±SD) of 67 (±8) years and mean FEV1 of 41 (610) % predicted. A total of 31% (n = 14) of patients had potentially pathogenic micro-organisms in quantitative bacterial cultures of samples obtained by protected specimen brush. Patients with COPD with positive cultures had lower levels of MUC2 both in BAL (P = 0.02) and in sputum (P = 0.01). No differences in MUC5B or MUC5AC levels were observed among the groups. Lower MUC2 levels were correlated with lower FEV1 (r = 0.32, P = 0.04) and higher sputum IL-6 (r =20.40, P = 0.01). Conclusions: Airway MUC2 levels are decreased in patients with severe COPD colonized by potentially pathogenic micro-organisms. These findings may indicate one of the mechanisms underlying airway colonization in patients with severe COPD.

Original languageEnglish (US)
Pages (from-to)636-642
Number of pages7
JournalAnnals of the American Thoracic Society
Volume13
Issue number5
DOIs
StatePublished - May 1 2016

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Mucin-2
Chronic Obstructive Pulmonary Disease
Mucins
Sputum
Bronchoalveolar Lavage
Bronchiectasis
Respiratory Function Tests
Bronchoscopy
Interleukin-6

Keywords

  • Airway infection
  • Airway inflammation
  • Chronic obstructive pulmonary disease
  • Mucins

ASJC Scopus subject areas

  • Medicine(all)
  • Pulmonary and Respiratory Medicine

Cite this

Sibila, O., Garcia-Bellmunt, L., Giner, J., Rodrigo-Troyano, A., Suarez-Cuartin, G., Torrego, A., ... Plaza, V. (2016). Airway Mucin 2 is decreased in patients with severe chronic obstructive pulmonary disease with bacterial colonization. Annals of the American Thoracic Society, 13(5), 636-642. https://doi.org/10.1513/AnnalsATS.201512-797OC

Airway Mucin 2 is decreased in patients with severe chronic obstructive pulmonary disease with bacterial colonization. / Sibila, Oriol; Garcia-Bellmunt, Laia; Giner, Jordi; Rodrigo-Troyano, Ana; Suarez-Cuartin, Guillermo; Torrego, Alfons; Castillo, Diego; Solanes, Ingrid; Mateus, Eder F.; Vidal, Silvia; Sanchez-Reus, Ferran; Sala, Ernest; Cosio, Borja G.; Restrepo, Marcos; Anzueto, Antonio R; Chalmers, James D.; Plaza, Vicente.

In: Annals of the American Thoracic Society, Vol. 13, No. 5, 01.05.2016, p. 636-642.

Research output: Contribution to journalArticle

Sibila, O, Garcia-Bellmunt, L, Giner, J, Rodrigo-Troyano, A, Suarez-Cuartin, G, Torrego, A, Castillo, D, Solanes, I, Mateus, EF, Vidal, S, Sanchez-Reus, F, Sala, E, Cosio, BG, Restrepo, M, Anzueto, AR, Chalmers, JD & Plaza, V 2016, 'Airway Mucin 2 is decreased in patients with severe chronic obstructive pulmonary disease with bacterial colonization', Annals of the American Thoracic Society, vol. 13, no. 5, pp. 636-642. https://doi.org/10.1513/AnnalsATS.201512-797OC
Sibila, Oriol ; Garcia-Bellmunt, Laia ; Giner, Jordi ; Rodrigo-Troyano, Ana ; Suarez-Cuartin, Guillermo ; Torrego, Alfons ; Castillo, Diego ; Solanes, Ingrid ; Mateus, Eder F. ; Vidal, Silvia ; Sanchez-Reus, Ferran ; Sala, Ernest ; Cosio, Borja G. ; Restrepo, Marcos ; Anzueto, Antonio R ; Chalmers, James D. ; Plaza, Vicente. / Airway Mucin 2 is decreased in patients with severe chronic obstructive pulmonary disease with bacterial colonization. In: Annals of the American Thoracic Society. 2016 ; Vol. 13, No. 5. pp. 636-642.
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abstract = "Rationale: Mucins are essential for airway defense against bacteria. We hypothesized that abnormal secreted airway mucin levels would be associated with bacterial colonization in patients with severe chronic obstructive pulmonary disease (COPD) Objectives: To investigate the relationship between mucin levels and the presence of potentially pathogenic micro-organisms in the airways of stable patients with severe COPD Methods: Clinically stable patients with severe COPD were examined prospectively. All patients underwent a computerized tomography scan, lung function tests, induced sputum collection, and bronchoscopy with bronchoalveolar lavage (BAL) and protected specimen brush. Patients with bronchiectasis were excluded. Secreted mucins (MUC2, MUC5AC, and MUC5B) and inflammatory markers were assessed in BAL and sputum by ELISA. Measurements and Main Results:Weenrolled 45 patients, with mean age (±SD) of 67 (±8) years and mean FEV1 of 41 (610) {\%} predicted. A total of 31{\%} (n = 14) of patients had potentially pathogenic micro-organisms in quantitative bacterial cultures of samples obtained by protected specimen brush. Patients with COPD with positive cultures had lower levels of MUC2 both in BAL (P = 0.02) and in sputum (P = 0.01). No differences in MUC5B or MUC5AC levels were observed among the groups. Lower MUC2 levels were correlated with lower FEV1 (r = 0.32, P = 0.04) and higher sputum IL-6 (r =20.40, P = 0.01). Conclusions: Airway MUC2 levels are decreased in patients with severe COPD colonized by potentially pathogenic micro-organisms. These findings may indicate one of the mechanisms underlying airway colonization in patients with severe COPD.",
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AU - Sibila, Oriol

AU - Garcia-Bellmunt, Laia

AU - Giner, Jordi

AU - Rodrigo-Troyano, Ana

AU - Suarez-Cuartin, Guillermo

AU - Torrego, Alfons

AU - Castillo, Diego

AU - Solanes, Ingrid

AU - Mateus, Eder F.

AU - Vidal, Silvia

AU - Sanchez-Reus, Ferran

AU - Sala, Ernest

AU - Cosio, Borja G.

AU - Restrepo, Marcos

AU - Anzueto, Antonio R

AU - Chalmers, James D.

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N2 - Rationale: Mucins are essential for airway defense against bacteria. We hypothesized that abnormal secreted airway mucin levels would be associated with bacterial colonization in patients with severe chronic obstructive pulmonary disease (COPD) Objectives: To investigate the relationship between mucin levels and the presence of potentially pathogenic micro-organisms in the airways of stable patients with severe COPD Methods: Clinically stable patients with severe COPD were examined prospectively. All patients underwent a computerized tomography scan, lung function tests, induced sputum collection, and bronchoscopy with bronchoalveolar lavage (BAL) and protected specimen brush. Patients with bronchiectasis were excluded. Secreted mucins (MUC2, MUC5AC, and MUC5B) and inflammatory markers were assessed in BAL and sputum by ELISA. Measurements and Main Results:Weenrolled 45 patients, with mean age (±SD) of 67 (±8) years and mean FEV1 of 41 (610) % predicted. A total of 31% (n = 14) of patients had potentially pathogenic micro-organisms in quantitative bacterial cultures of samples obtained by protected specimen brush. Patients with COPD with positive cultures had lower levels of MUC2 both in BAL (P = 0.02) and in sputum (P = 0.01). No differences in MUC5B or MUC5AC levels were observed among the groups. Lower MUC2 levels were correlated with lower FEV1 (r = 0.32, P = 0.04) and higher sputum IL-6 (r =20.40, P = 0.01). Conclusions: Airway MUC2 levels are decreased in patients with severe COPD colonized by potentially pathogenic micro-organisms. These findings may indicate one of the mechanisms underlying airway colonization in patients with severe COPD.

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