TY - JOUR
T1 - Aging does not reduce heat shock protein 70 in the absence of chronic insulin resistance
AU - Kavanagh, Kylie
AU - Wylie, Ashley T.
AU - Chavanne, Tara J.
AU - Jorgensen, Matthew J.
AU - Voruganti, V. Saroja
AU - Comuzzie, Anthony G.
AU - Kaplan, Jay R.
AU - McCall, Charles E.
AU - Kritchevsky, Stephen B.
N1 - Funding Information:
This study was funded in part by National Institutes of Health grants K01AG 033641 P40 RR 019963 , and R01HL 024736 and by the Wake Forest University Claude D. Pepper Older Americans Independence Center (P30AG 021332 ).
PY - 2012/10
Y1 - 2012/10
N2 - Heat shock protein (HSP)70 decreases with age. Often aging is associated with coincident insulin resistance and higher blood glucose levels, which also associate with lower HSP70. We aimed to understand how these factors interrelate through a series of experiments using vervet monkeys (Chlorocebus aethiops sabaeous). Monkeys (n = 284, 4-25 years) fed low-fat diets showed no association of muscle HSP70 with age (r =. 04, p =. 53), but levels were highly heritable. Insulin resistance was induced in vervet monkeys with high-fat diets, and muscle biopsies were taken after 0.3 or 6 years. HSP70 levels were significantly greater after 0.3 years (+72%, p <. 05) but were significantly lower following 6 years of high-fat diet (-77%, p <. 05). Associations with glucose also switched from being positive (r =. 44, p =. 03) to strikingly negative (r = -.84, p <. 001) with increasing insulin resistance. In conclusion, a low-fat diet may preserve tissue HSP70 and health with aging, whereas high-fat diets, insulin resistance, and genetic factors may be more important than age for determining HSP70 levels.
AB - Heat shock protein (HSP)70 decreases with age. Often aging is associated with coincident insulin resistance and higher blood glucose levels, which also associate with lower HSP70. We aimed to understand how these factors interrelate through a series of experiments using vervet monkeys (Chlorocebus aethiops sabaeous). Monkeys (n = 284, 4-25 years) fed low-fat diets showed no association of muscle HSP70 with age (r =. 04, p =. 53), but levels were highly heritable. Insulin resistance was induced in vervet monkeys with high-fat diets, and muscle biopsies were taken after 0.3 or 6 years. HSP70 levels were significantly greater after 0.3 years (+72%, p <. 05) but were significantly lower following 6 years of high-fat diet (-77%, p <. 05). Associations with glucose also switched from being positive (r =. 44, p =. 03) to strikingly negative (r = -.84, p <. 001) with increasing insulin resistance. In conclusion, a low-fat diet may preserve tissue HSP70 and health with aging, whereas high-fat diets, insulin resistance, and genetic factors may be more important than age for determining HSP70 levels.
KW - Aging
KW - Heat shock protein 70
KW - Insulin resistance
KW - Nonhuman primate
KW - Western diet
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U2 - 10.1093/gerona/gls008
DO - 10.1093/gerona/gls008
M3 - Article
C2 - 22403054
AN - SCOPUS:84866078990
VL - 67 A
SP - 1014
EP - 1021
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
SN - 1079-5006
IS - 10
ER -