Abstract
Heat shock protein (HSP)70 decreases with age. Often aging is associated with coincident insulin resistance and higher blood glucose levels, which also associate with lower HSP70. We aimed to understand how these factors interrelate through a series of experiments using vervet monkeys (Chlorocebus aethiops sabaeous). Monkeys (n = 284, 4-25 years) fed low-fat diets showed no association of muscle HSP70 with age (r =. 04, p =. 53), but levels were highly heritable. Insulin resistance was induced in vervet monkeys with high-fat diets, and muscle biopsies were taken after 0.3 or 6 years. HSP70 levels were significantly greater after 0.3 years (+72%, p <. 05) but were significantly lower following 6 years of high-fat diet (-77%, p <. 05). Associations with glucose also switched from being positive (r =. 44, p =. 03) to strikingly negative (r = -.84, p <. 001) with increasing insulin resistance. In conclusion, a low-fat diet may preserve tissue HSP70 and health with aging, whereas high-fat diets, insulin resistance, and genetic factors may be more important than age for determining HSP70 levels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1014-1021 |
| Number of pages | 8 |
| Journal | Journals of Gerontology - Series A Biological Sciences and Medical Sciences |
| Volume | 67 A |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2012 |
| Externally published | Yes |
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Keywords
- Aging
- Heat shock protein 70
- Insulin resistance
- Nonhuman primate
- Western diet
ASJC Scopus subject areas
- Aging
- Geriatrics and Gerontology
- Medicine(all)
Cite this
Aging does not reduce heat shock protein 70 in the absence of chronic insulin resistance. / Kavanagh, Kylie; Wylie, Ashley T.; Chavanne, Tara J.; Jorgensen, Matthew J.; Voruganti, V. Saroja; Comuzzie, Anthony G.; Kaplan, Jay R.; McCall, Charles E.; Kritchevsky, Stephen B.
In: Journals of Gerontology - Series A Biological Sciences and Medical Sciences, Vol. 67 A, No. 10, 10.2012, p. 1014-1021.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aging does not reduce heat shock protein 70 in the absence of chronic insulin resistance
AU - Kavanagh, Kylie
AU - Wylie, Ashley T.
AU - Chavanne, Tara J.
AU - Jorgensen, Matthew J.
AU - Voruganti, V. Saroja
AU - Comuzzie, Anthony G.
AU - Kaplan, Jay R.
AU - McCall, Charles E.
AU - Kritchevsky, Stephen B.
PY - 2012/10
Y1 - 2012/10
N2 - Heat shock protein (HSP)70 decreases with age. Often aging is associated with coincident insulin resistance and higher blood glucose levels, which also associate with lower HSP70. We aimed to understand how these factors interrelate through a series of experiments using vervet monkeys (Chlorocebus aethiops sabaeous). Monkeys (n = 284, 4-25 years) fed low-fat diets showed no association of muscle HSP70 with age (r =. 04, p =. 53), but levels were highly heritable. Insulin resistance was induced in vervet monkeys with high-fat diets, and muscle biopsies were taken after 0.3 or 6 years. HSP70 levels were significantly greater after 0.3 years (+72%, p <. 05) but were significantly lower following 6 years of high-fat diet (-77%, p <. 05). Associations with glucose also switched from being positive (r =. 44, p =. 03) to strikingly negative (r = -.84, p <. 001) with increasing insulin resistance. In conclusion, a low-fat diet may preserve tissue HSP70 and health with aging, whereas high-fat diets, insulin resistance, and genetic factors may be more important than age for determining HSP70 levels.
AB - Heat shock protein (HSP)70 decreases with age. Often aging is associated with coincident insulin resistance and higher blood glucose levels, which also associate with lower HSP70. We aimed to understand how these factors interrelate through a series of experiments using vervet monkeys (Chlorocebus aethiops sabaeous). Monkeys (n = 284, 4-25 years) fed low-fat diets showed no association of muscle HSP70 with age (r =. 04, p =. 53), but levels were highly heritable. Insulin resistance was induced in vervet monkeys with high-fat diets, and muscle biopsies were taken after 0.3 or 6 years. HSP70 levels were significantly greater after 0.3 years (+72%, p <. 05) but were significantly lower following 6 years of high-fat diet (-77%, p <. 05). Associations with glucose also switched from being positive (r =. 44, p =. 03) to strikingly negative (r = -.84, p <. 001) with increasing insulin resistance. In conclusion, a low-fat diet may preserve tissue HSP70 and health with aging, whereas high-fat diets, insulin resistance, and genetic factors may be more important than age for determining HSP70 levels.
KW - Aging
KW - Heat shock protein 70
KW - Insulin resistance
KW - Nonhuman primate
KW - Western diet
UR - http://www.scopus.com/inward/record.url?scp=84866078990&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866078990&partnerID=8YFLogxK
U2 - 10.1093/gerona/gls008
DO - 10.1093/gerona/gls008
M3 - Article
C2 - 22403054
AN - SCOPUS:84866078990
VL - 67 A
SP - 1014
EP - 1021
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
SN - 1079-5006
IS - 10
ER -