Age-related changes in isolated rat hepatocytes. Comparison of size, morphology, binucleation, and protein content

G. L. Engelmann, A. Richardson, A. Katz, J. A. Fierer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Hepatocytes isolated from 6-, 12-, 18-, and 30-month-old female Fischer F344 rats were examined by scanning electron microscopy. No significant change in cell size with age was observed. However, the surface morphology of the cells isolated from the older animals exhibited a significant increase in surface folds. This feature did not exceed 10% of the cell population until 12 months of age and continued to increase to 31% of the cells in 30-month-old rats. From 6 to 12 months of age, there was a significant increase in protein content of the hepatocytes. No further increase in protein content occurred during senescence. An increase in percentage of binuclear cells occurred after 24 months of age. Because ploidy and binucleation increase with increasing age, it appears that the nuclear/cytoplasmic ratio changes as a function of age.

Original languageEnglish (US)
Pages (from-to)385-395
Number of pages11
JournalMechanisms of Ageing and Development
Volume16
Issue number4
DOIs
StatePublished - 1981
Externally publishedYes

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Rats
Hepatocytes
Inbred F344 Rats
Surface morphology
Animals
Proteins
Cells
Scanning electron microscopy
Ploidies
Cell Size
Electron Scanning Microscopy
Population

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Age-related changes in isolated rat hepatocytes. Comparison of size, morphology, binucleation, and protein content. / Engelmann, G. L.; Richardson, A.; Katz, A.; Fierer, J. A.

In: Mechanisms of Ageing and Development, Vol. 16, No. 4, 1981, p. 385-395.

Research output: Contribution to journalArticle

Engelmann, G. L. ; Richardson, A. ; Katz, A. ; Fierer, J. A. / Age-related changes in isolated rat hepatocytes. Comparison of size, morphology, binucleation, and protein content. In: Mechanisms of Ageing and Development. 1981 ; Vol. 16, No. 4. pp. 385-395.
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