Age-related base excision repair activity in mouse brain and liver nuclear extracts

Gabriel W. Intano, Eun Ju Cho, C. Alex McMahan, Christi A. Walter

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

To assess DNA repair activity relative to age, in vitro base excision repair assays were performed using brain and liver nuclear extracts prepared from mice of various ages. An 85% decline in repair activity was observed in brain nuclear extracts and a 50% decrease in liver nuclear extracts prepared from old mice compared with 6-day-old mice. Brain nuclear extracts prepared from old mice showed a decreased abundance of DNA polymerase-β, but the addition of purified protein did not restore base excision repair activity. Abundances of other tested base excision repair proteins did not change relative to age. The conclusion is that, during aging, a decline in DNA repair could contribute to increased levels of DNA damage and mutagenesis.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalJournals of Gerontology - Series A Biological Sciences and Medical Sciences
Volume58
Issue number3
DOIs
StatePublished - Mar 1 2003

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

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