Age-Associated Decline in Thymic B Cell Expression of Aire and Aire-Dependent Self-Antigens

Sergio Cepeda, Carolina Cantu, Stephanie Orozco, Yangming Xiao, Zoe Brown, Manpreet K. Semwal, Thomas Venables, Mark S. Anderson, Ann V Griffith

Research output: Contribution to journalArticle

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Abstract

Although autoimmune disorders are a significant source of morbidity and mortality in older individuals, the mechanisms governing age-associated increases in susceptibility remain incompletely understood. Central T cell tolerance is mediated through presentation of self-antigens by cells constituting the thymic microenvironment, including epithelial cells, dendritic cells, and B cells. Medullary thymic epithelial cells (mTECs) and B cells express distinct cohorts of self-antigens, including tissue-restricted self-antigens (TRAs), such that developing T cells are tolerized to antigens from peripheral tissues. We find that expression of the TRA transcriptional regulator Aire, as well as Aire-dependent genes, declines with age in thymic B cells in mice and humans and that cell-intrinsic and cell-extrinsic mechanisms contribute to the diminished capacity of peripheral B cells to express Aire within the thymus. Our findings indicate that aging may diminish the ability of thymic B cells to tolerize T cells, revealing a potential mechanistic link between aging and autoimmunity. Mechanisms governing age-associated increases in autoimmunity remain elusive. Expression of Aire and downstream self-antigens by thymic B cells helps tolerize developing T cells. Cepeda et al. report age-associated declines in expression of Aire and self-antigen genes in thymic B cells concomitant with increases in T-bet and IgG2a expression.

LanguageEnglish (US)
Pages1276-1287
Number of pages12
JournalCell Reports
Volume22
Issue number5
DOIs
StatePublished - Jan 30 2018
Externally publishedYes

Fingerprint

Autoantigens
Thymus Gland
B-Lymphocytes
T-Lymphocytes
Autoimmunity
Epithelial Cells
Dendritic Cells
Genes
Morbidity
Antigens
Mortality

Keywords

  • aging
  • Aire
  • B cell
  • thymus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Age-Associated Decline in Thymic B Cell Expression of Aire and Aire-Dependent Self-Antigens. / Cepeda, Sergio; Cantu, Carolina; Orozco, Stephanie; Xiao, Yangming; Brown, Zoe; Semwal, Manpreet K.; Venables, Thomas; Anderson, Mark S.; Griffith, Ann V.

In: Cell Reports, Vol. 22, No. 5, 30.01.2018, p. 1276-1287.

Research output: Contribution to journalArticle

Cepeda, S, Cantu, C, Orozco, S, Xiao, Y, Brown, Z, Semwal, MK, Venables, T, Anderson, MS & Griffith, AV 2018, 'Age-Associated Decline in Thymic B Cell Expression of Aire and Aire-Dependent Self-Antigens' Cell Reports, vol. 22, no. 5, pp. 1276-1287. https://doi.org/10.1016/j.celrep.2018.01.015
Cepeda, Sergio ; Cantu, Carolina ; Orozco, Stephanie ; Xiao, Yangming ; Brown, Zoe ; Semwal, Manpreet K. ; Venables, Thomas ; Anderson, Mark S. ; Griffith, Ann V. / Age-Associated Decline in Thymic B Cell Expression of Aire and Aire-Dependent Self-Antigens. In: Cell Reports. 2018 ; Vol. 22, No. 5. pp. 1276-1287.
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