Age and food restriction alter the porphyrin concentration and mRNA levels for 5-aminolevulinate synthase in rat harderian gland

Carmen Rodriguez, Armando Menendez-Pelaez, Kimberly A. Howes, Russel J. Reiter

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The effects of age and food restriction on the porphyrin concentration in Harderian glands were studied in male Fisher 344 rats. Harderian gland porphyrin concentrations increased with age; this was statistically significant in 20 month old aninals compared with 3 month old animals. Food restriction (by 40%) prevented the age-associated rise in porphyrins; thus, in 20 month old food restricted rats had porphyrin concentrations similar to those found in young animals. In a second experiment, we correlated the age-associated rise in Harderian gland porphyrin concentrations with an increase in mRNA levels for 5-aminolevulinate synthase (ALV-S). Both the porphyrin concentration and ALV-S mRNA rose at 12 and 18 months of age, but decreased by 24 months of age. It is concluded that, a) porphyrin biosynthesis in the Harderian glands increases up to 20 months of age but decreases in rats that are 24 months old, and b) food restriction prevents the porphyrin rise associated with age in the Harderian gland of male Fisher 344 rats.

Original languageEnglish (US)
Pages (from-to)1891-1897
Number of pages7
JournalLife Sciences
Volume51
Issue number24
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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