Agarose gel electrophoresis of bacteriophages and related particles. II. Correction of electrophoretic mobilities for the electro‐osmosis of agarose

Philip Serwer, Shirley J. Hayes

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Bacteriophage T4, bacteriophage T4 without its tail fibers, and bacteriophage P22 without its tail have been subjected to electrophoresis in agarose gels; electrophoretic mobility (μ) of these particles as a function of the concentration of agarose (A) has been determined using preparations of agarose with different amounts of electro‐osmosis. When μ was extrapolated to an A of 0 (μo), it was found that a term, μE, constant for each preparation of agarose must be added to μo to correct for electro‐osmotic flow. A relationship between μE and a manufacturer‐determined coefficient of electro‐osmosis has been empirically derived. Corrected μo's are in agreement with data obtained by solid support‐free electrophoresis.

Original languageEnglish (US)
Pages (from-to)80-85
Number of pages6
JournalElectrophoresis
Volume3
Issue number2
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Agarose gel electrophoresis of bacteriophages and related particles. II. Correction of electrophoretic mobilities for the electro‐osmosis of agarose'. Together they form a unique fingerprint.

Cite this