Agarose gel electrophoresis of bacteriophages and related particles. IV. An improved procedure for determining the size of spherical particles

Philip Serwer, Jerry L. Allen

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Abstract

Attempts have been made to improve the accuracy with which sieving during electrophoresis in gels can be used to determine the size of viruses and related particles. Using as samples spherical bacteriophages and related particles of known radius (13.3–41.9 nm), it was found that electrophoretic mobility (μ) was a linear function of agarose concentration (A) in a hydroxyethylated agarose (SeaPlaque, Marine Colloids) for 0.4 ⩽ A ⩽ 1.6. The value of A extrapolated to a μ of 0 (A0) was a decreasing function of particle outer radius and was independent of the solid support‐free μ. Procedures for determining outer particle radius from values of A0 have been developed. When used with internal standards, these procedures measure outer particle radius with an accuracy of ±4 %.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalElectrophoresis
Volume4
Issue number4
DOIs
StatePublished - 1983

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ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

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