Affinity chromatography of RNase inhibitor

Melissa A. Garcia, Robert J. Klebe

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Of the reagents used in reverse transcription-PCR (RT-PCR), RNase Inhibitor is the most costly on a per assay basis. A simple method for purification of RNase Inhibitor from bovine liver is described. Approximately 40 000 units of RNase Inhibitor can be purified to homogeneity from 400 g of bovine liver within two days using inexpensive reagents. The method described employs (a) facile procedures to rapidly obtain a clarified liver extract, (b) affinity chromatography of RNase Inhibitor on RNase-A-Sepharose, and (c) batchwise concentration of the purified protein with a molecular sieve resin.

Original languageEnglish (US)
Pages (from-to)231-233
Number of pages3
JournalMolecular Biology Reports
Volume24
Issue number4
DOIs
StatePublished - Nov 1997
Externally publishedYes

Keywords

  • PCR
  • Protein
  • Purification
  • RNA
  • RNase Inhibitor
  • RT-PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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