Advanced shotgun lipidomics for characterization of altered lipid patterns in neurodegenerative diseases and brain injury

Miao Wang, Xianlin Han

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Multi-dimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) is a powerful technology platform among current lipidomics practices due to its high efficiency, sensitivity, and reproducibility, as well as its broad coverage. This platform has been widely used to determine the altered lipid profiles induced by diseases, injury, genetic manipulations, drug treatments, and aging, among others. Herein, we summarize the principles underlying this platform and present a protocol for analysis of many of the lipid classes and subclasses covered by MDMS-SL directly from lipid extracts of brain samples. We believe that this protocol can aid researchers in the field to determine altered lipid patterns in neurodegenerative diseases and brain injury.

Original languageEnglish (US)
Pages (from-to)405-422
Number of pages18
JournalMethods in Molecular Biology
Volume1303
DOIs
StatePublished - 2016
Externally publishedYes

Keywords

  • Alzheimer’s disease
  • Brain injury
  • Lipidome
  • MDMS-SL
  • Mass spectrometry
  • Metabolome
  • Metabolomics
  • Multi-dimensional mass spectrometry (MS)-based shotgun lipidomics
  • Neurodegeneration
  • Shotgun lipidomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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