TY - JOUR
T1 - Adult SVZ Stem Cells Lie in a Vascular Niche
T2 - A Quantitative Analysis of Niche Cell-Cell Interactions
AU - Shen, Qin
AU - Wang, Yue
AU - Kokovay, Erzsebet
AU - Lin, Gang
AU - Chuang, Shu Mien
AU - Goderie, Susan K.
AU - Roysam, Badrinath
AU - Temple, Sally
N1 - Funding Information:
We thank Rebecca Stern for assistance with the summary figure, Patricia Lederman and Cindy Butler for technical help, and Adreinne Dorr and Michael Dipersio for discussions on the SVZ vasculature. We are grateful for antibodies from Drs. Charles Stiles, David Anderson, and Jeffrey Miner. This project was supported by NINDS through NIH grant R01NS051531, NYS contract C#022044, and the Regenerative Research Foundation. E.K. was supported by NIDA grant 5T32DA007307. Portions of this work were supported by the Center for Subsurface Sensing and Imaging Systems, NSF Engineering Research Centers Program (Award Number EEC-9986821), and Rensselaer Polytechnic Institute.
PY - 2008/9/11
Y1 - 2008/9/11
N2 - There is an emerging understanding of the importance of the vascular system within stem cell niches. Here, we examine whether neural stem cells (NSCs) in the adult subventricular zone (SVZ) lie close to blood vessels, using three-dimensional whole mounts, confocal microscopy, and automated computer-based image quantification. We found that the SVZ contains a rich plexus of blood vessels that snake along and within neuroblast chains. Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. Apical GFAP+ cells are admixed within the ependymal layer and some span between the ventricle and blood vessels, occupying a specialized microenvironment. Adult SVZ progenitor cells express the laminin receptor α6β1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating that it plays a functional role in binding SVZ stem cells within the vascular niche.
AB - There is an emerging understanding of the importance of the vascular system within stem cell niches. Here, we examine whether neural stem cells (NSCs) in the adult subventricular zone (SVZ) lie close to blood vessels, using three-dimensional whole mounts, confocal microscopy, and automated computer-based image quantification. We found that the SVZ contains a rich plexus of blood vessels that snake along and within neuroblast chains. Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. Apical GFAP+ cells are admixed within the ependymal layer and some span between the ventricle and blood vessels, occupying a specialized microenvironment. Adult SVZ progenitor cells express the laminin receptor α6β1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating that it plays a functional role in binding SVZ stem cells within the vascular niche.
KW - STEMCELL
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U2 - 10.1016/j.stem.2008.07.026
DO - 10.1016/j.stem.2008.07.026
M3 - Article
C2 - 18786416
AN - SCOPUS:50849142558
SN - 1934-5909
VL - 3
SP - 289
EP - 300
JO - Cell Stem Cell
JF - Cell Stem Cell
IS - 3
ER -