Adult SVZ Stem Cells Lie in a Vascular Niche: A Quantitative Analysis of Niche Cell-Cell Interactions

Qin Shen, Yue Wang, Erzsebet K Cearley, Gang Lin, Shu Mien Chuang, Susan K. Goderie, Badrinath Roysam, Sally Temple

Research output: Contribution to journalArticle

702 Citations (Scopus)

Abstract

There is an emerging understanding of the importance of the vascular system within stem cell niches. Here, we examine whether neural stem cells (NSCs) in the adult subventricular zone (SVZ) lie close to blood vessels, using three-dimensional whole mounts, confocal microscopy, and automated computer-based image quantification. We found that the SVZ contains a rich plexus of blood vessels that snake along and within neuroblast chains. Cells expressing stem cell markers, including GFAP, and proliferation markers are closely apposed to the laminin-containing extracellular matrix (ECM) surrounding vascular endothelial cells. Apical GFAP+ cells are admixed within the ependymal layer and some span between the ventricle and blood vessels, occupying a specialized microenvironment. Adult SVZ progenitor cells express the laminin receptor α6β1 integrin, and blocking this inhibits their adhesion to endothelial cells, altering their position and proliferation in vivo, indicating that it plays a functional role in binding SVZ stem cells within the vascular niche.

Original languageEnglish (US)
Pages (from-to)289-300
Number of pages12
JournalCell Stem Cell
Volume3
Issue number3
DOIs
StatePublished - Sep 11 2008
Externally publishedYes

Fingerprint

Lateral Ventricles
Cell Communication
Blood Vessels
Stem Cells
Endothelial Cells
Laminin Receptors
Stem Cell Niche
Neural Stem Cells
Snakes
Laminin
Confocal Microscopy
Integrins
Extracellular Matrix

Keywords

  • STEMCELL

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Medicine
  • Genetics

Cite this

Adult SVZ Stem Cells Lie in a Vascular Niche : A Quantitative Analysis of Niche Cell-Cell Interactions. / Shen, Qin; Wang, Yue; Cearley, Erzsebet K; Lin, Gang; Chuang, Shu Mien; Goderie, Susan K.; Roysam, Badrinath; Temple, Sally.

In: Cell Stem Cell, Vol. 3, No. 3, 11.09.2008, p. 289-300.

Research output: Contribution to journalArticle

Shen, Qin ; Wang, Yue ; Cearley, Erzsebet K ; Lin, Gang ; Chuang, Shu Mien ; Goderie, Susan K. ; Roysam, Badrinath ; Temple, Sally. / Adult SVZ Stem Cells Lie in a Vascular Niche : A Quantitative Analysis of Niche Cell-Cell Interactions. In: Cell Stem Cell. 2008 ; Vol. 3, No. 3. pp. 289-300.
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