TY - JOUR
T1 - Adult svz lineage cells home to and leave the vascular niche via differential responses to SDF1/CXCR4 signaling
AU - Kokovay, Erzsebet
AU - Goderie, Susan
AU - Wang, Yue
AU - Lotz, Steve
AU - Lin, Gang
AU - Sun, Yu
AU - Roysam, Badrinath
AU - Shen, Qin
AU - Temple, Sally
N1 - Funding Information:
We thank Chris Fasano and Tim Phoenix for assistance in generating lentiviral shRNA contructs and Patty Lederman for invaluable technical support. This project was supported by NIH grant R01NS051531 and the Regenerative Research Foundation, and E.K. was supported in part by NIDA grant 5T32DA007307.
PY - 2010/8/6
Y1 - 2010/8/6
N2 - Neural progenitor cells (NPCs) in the adult subventricular zone (SVZ) are associated with ependymal and vasculature niches, which regulate stem cell self-renewal and differentiation. Activated Type B stem cells and their progeny, the transit-amplifying type C cells, which express EGFR, are most highly associated with vascular cells, indicating that this niche supports lineage progression. Here, we show that proliferative SVZ progenitor cells home to endothelial cells in a stromal-derived factor 1 (SDF1)- and CXC chemokine receptor 4 (CXCR4)-dependent manner. We show that SDF1 strongly upregulates EGFR and α6 integrin in activated type B and type C cells, enhancing their activated state and their ability to bind laminin in the vascular niche. SDF1 increases the motility of type A neuroblasts, which migrate from the SVZ toward the olfactory bulb. Thus, differential responses to SDF1 can regulate progenitor cell occupancy of and exit from the adult SVZ vascular niche.
AB - Neural progenitor cells (NPCs) in the adult subventricular zone (SVZ) are associated with ependymal and vasculature niches, which regulate stem cell self-renewal and differentiation. Activated Type B stem cells and their progeny, the transit-amplifying type C cells, which express EGFR, are most highly associated with vascular cells, indicating that this niche supports lineage progression. Here, we show that proliferative SVZ progenitor cells home to endothelial cells in a stromal-derived factor 1 (SDF1)- and CXC chemokine receptor 4 (CXCR4)-dependent manner. We show that SDF1 strongly upregulates EGFR and α6 integrin in activated type B and type C cells, enhancing their activated state and their ability to bind laminin in the vascular niche. SDF1 increases the motility of type A neuroblasts, which migrate from the SVZ toward the olfactory bulb. Thus, differential responses to SDF1 can regulate progenitor cell occupancy of and exit from the adult SVZ vascular niche.
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U2 - 10.1016/j.stem.2010.05.019
DO - 10.1016/j.stem.2010.05.019
M3 - Article
C2 - 20682445
AN - SCOPUS:77956241246
VL - 7
SP - 163
EP - 173
JO - Cell Stem Cell
JF - Cell Stem Cell
SN - 1934-5909
IS - 2
ER -