Adrenergic regulation of the intracellular [Ca2+] and voltage-operated Ca2+ channel currents in the rat prostate neuroendocrine cells

Jun Hee Kim, Sun Young Shin, Joo Hyun Nam, Eun Kyung Hong, Young Shin Chung, Jeong Yun Jeong, Jeongyoon Kang, Dae Yong Uhm, Sung Joon Kim

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

BACKGROUND. The prostate gland contains numerous neuroendocrine cells (PNECs) innervated by adrenergic neurons. PNECs are believed to influence the growth and physiological function of the prostate gland via paracrine release of hormones. MATERIALS AND METHODS. Using fura-2 fluorescence measurement and patch-clamp techniques, we investigated the effects of adrenergic stimulation on cytosolic concentration of Ca2+ ([Ca2+]c) and high voltage-activated Ca2+ channel currents (HVA-I Ca) of the putative rat prostate neuroendocrine cells (RPNECs) freshly isolated by an enzymic digestion. RESULTS. Noradrenaline (NA, 1 μM) induced a sharp, transient increase of [Ca2+]c measured by the fura-2 fluorescence. Pharmacological studies showed that α1-adrenoceptors (α1-ARs) coupled with PLC/IP3 signaling pathway induce the release of stored Ca2+, which subsequently recruits store-operated Ca2+ entry pathways. In the whole-cell voltage clamp experiment, NA decreased the amplitude of HVA-ICa by 40%, which was mimicked by an α2-AR agonist (UK14304) but not by an α1-AR agonist (phenyleprine). After selective blockade of N-type Ca 2+ channels by ω-conotoxin GVIA, the addition of NA showed no further inhibition on the remaining L-type Ca2+ channel currents. The adrenergic inhibition of HVA-ICa was partially prevented by the pretreatment with pertussis toxin (PTX) (5 pg/ml, 4 hr, 37°C). CONCLUSIONS. RPNECs express both α1- and α2-ARs, signaling the release of stored Ca2+ and the inhibition of N-type Ca2+ channels, respectively.

Original languageEnglish (US)
Pages (from-to)99-110
Number of pages12
JournalProstate
Volume57
Issue number2
DOIs
StatePublished - Oct 1 2003
Externally publishedYes

Keywords

  • Adrenoceptors, voltage-activated Ca channel
  • Intracellular Ca concentration
  • Neuroendocrine cell
  • Prostate

ASJC Scopus subject areas

  • Oncology
  • Urology

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    Kim, J. H., Shin, S. Y., Nam, J. H., Hong, E. K., Chung, Y. S., Jeong, J. Y., Kang, J., Uhm, D. Y., & Kim, S. J. (2003). Adrenergic regulation of the intracellular [Ca2+] and voltage-operated Ca2+ channel currents in the rat prostate neuroendocrine cells. Prostate, 57(2), 99-110. https://doi.org/10.1002/pros.10277