Radioligand binding assays were used to demonstrate the presence of α1, α2 and β receptors in rat spinal cord Specific and saturable binding was exhibited for [3H]-WB 4101 (α1)-aminoclonidlne (α2) and [3H]-dihydroalprenolol (β). Binding was of high affinity and the total number of binding sites (Bmax) were: α, 66.5 ± 1.0 fmol/mg protein; α2, 20.0 ± 0.6 fmol/mg protein; β, 10.2 ± 0.3 fmol/mg protein. The data confirms the existence of adrenergic receptors in spinal cord and provides further evidence of the role of catecholaminergic neurons in regulating spinal cord physiology.
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience