Radioligand binding assays were used to demonstrate the presence of α1, α(2) and β receptora in rat spinal cord. Specific and saturable binding was exhibited for [3H-WB 4101 (α1), [3H]-aminoclonldine (α2) and [3H]-dihydroalprenolol(β). Binding was of high affinity and the total number of binding sites (Bmaxwere: α1, 66.5 ± 1.0 fmol/mg protein; α2, 20.0 ± 0.6 fmol/mg protein; β, 10.2 ± 0.3 fmol/mg protein. The data confirms the existence of adrenergic receptors in spinal cord and provides further evidence of the role of catecholaminergic neurons in regulating spinal cord physiology.
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience