Adjustable ellipsoid nanoparticles assembled from re-engineered connectors of the bacteriophage Phi29 DNA packaging motor

Feng Xiao, Ying Cai, Joseph Che Yen Wang, Dominik Green, R. Holland Cheng, Borries Demeler, Peixuan Guo

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A 24 × 30 nm ellipsoid nanoparticle containing 84 subunits or 7 dodecamers of the re-engineered core protein of the bacteriophage phi29 packaging motor was constructed. Homogeneous nanoparticles were obtained with simple one-step purification. Electron microscopy and analytical ultracentrifugation were employed to elucidate the structure, shape, size, and mechanism of assembly. The formation of this structure was mediated and stabilized by N-terminal peptide extensions. Reversal of the 84-subunit ellipsoid nanoparticle to its dodecamer subunit was controlled by the cleavage of the extended N-terminal peptide with a protease. The 84 outward-oriented C-termini were conjugated with a streptavidin binding peptide which can be used for the incorporation of markers. This further extends the application of this nanoparticle to pathogen detection and disease diagnosis by signal enhancement.

Original languageEnglish (US)
Pages (from-to)2163-2170
Number of pages8
JournalACS Nano
Volume3
Issue number8
DOIs
StatePublished - Aug 25 2009

Keywords

  • Bacteriophage phi29 connector
  • Bionanotechnology
  • Nanobiotechnology
  • Phi29 DNA packaging motor
  • Protein nanoparticles
  • Viral DNA packaging
  • Virus assemble

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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