Adiponectin regulates bone marrow mesenchymal stem cell niche through a unique signal transduction pathway: An approach for treating bone disease in diabetes

Liming Yu, Qisheng Tu, Qianqian Han, Lan Zhang, Lei Sui, Leilei Zheng, Shu Meng, Yin Tang, Dongying Xuan, Jin Zhang, Dana Murray, Qingping Shen, Jessica Cheng, Sung Hoon Kim, Lily Q Dong, Paloma Valverde, Xinming Cao, Jake Chen

Research output: Contribution to journalArticle

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Abstract

Adiponectin (APN) is an adipocyte-secreted adipokine that exerts well-characterized antidiabetic properties. Patients with type 2 diabetes (T2D) are characterized by reduced APN levels in circulation and impaired stem cell and progenitor cell mobilization from the bone marrow for tissue repair and remodeling. In this study, we found that APN regulates the mobilization and recruitment of bone marrow-derived mesenchymal stem cells (BMSCs) to participate in tissue repair and regeneration. APN facilitated BMSCs migrating from the bone marrow into the circulation to regenerate bone by regulating stromal cell-derived factor (SDF)-1 in a mouse bone defect model. More importantly, we found that systemic APN infusion ameliorated diabetic mobilopathy of BMSCs, lowered glucose concentration, and promoted bone regeneration in diet-induced obesity mice. In vitro studies allowed us to identify Smad1/5/8 as a novel signaling mediator of APN receptor (AdipoR)-1 in BMSCs and osteoblasts. APN stimulation of MC3T3-E1 osteoblastic cells led to Smad1/5/8 phosphorylation and nuclear localization and increased SDF-1 mRNA expression. Although APN-mediated phosphorylation of Smad1/5/8 occurred independently from adaptor protein, phosphotyrosine interaction, pleckstrin homology domain, and leucine zipper containing 1, it correlated with the disassembly of protein kinase casein kinase 2 and AdipoR1 in immunoprecipitation experiments. Taken together, this study identified APN as a regulator of BMSCs migration in response to bone injury. Therefore, our findings suggest APN signaling could be a potential therapeutic target to improve bone regeneration and homeostasis, especially in obese and T2D patients. Stem Cells 2015;33:240-252

Original languageEnglish (US)
Pages (from-to)240-252
Number of pages13
JournalStem Cells
Volume33
Issue number1
DOIs
StatePublished - Jan 1 2015

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Stem Cell Niche
Bone Diseases
Adiponectin
Mesenchymal Stromal Cells
Signal Transduction
Bone Marrow
Chemokine CXCL12
Bone and Bones
Bone Regeneration
Stem Cells
Type 2 Diabetes Mellitus
Adiponectin Receptors
Phosphorylation
Casein Kinase II
Leucine Zippers
Phosphotyrosine
Adipokines
Osteoblasts
Immunoprecipitation
Hypoglycemic Agents

Keywords

  • Adiponectin
  • Bone regeneration
  • Cell mobilization
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Adiponectin regulates bone marrow mesenchymal stem cell niche through a unique signal transduction pathway : An approach for treating bone disease in diabetes. / Yu, Liming; Tu, Qisheng; Han, Qianqian; Zhang, Lan; Sui, Lei; Zheng, Leilei; Meng, Shu; Tang, Yin; Xuan, Dongying; Zhang, Jin; Murray, Dana; Shen, Qingping; Cheng, Jessica; Kim, Sung Hoon; Dong, Lily Q; Valverde, Paloma; Cao, Xinming; Chen, Jake.

In: Stem Cells, Vol. 33, No. 1, 01.01.2015, p. 240-252.

Research output: Contribution to journalArticle

Yu, L, Tu, Q, Han, Q, Zhang, L, Sui, L, Zheng, L, Meng, S, Tang, Y, Xuan, D, Zhang, J, Murray, D, Shen, Q, Cheng, J, Kim, SH, Dong, LQ, Valverde, P, Cao, X & Chen, J 2015, 'Adiponectin regulates bone marrow mesenchymal stem cell niche through a unique signal transduction pathway: An approach for treating bone disease in diabetes', Stem Cells, vol. 33, no. 1, pp. 240-252. https://doi.org/10.1002/stem.1844
Yu, Liming ; Tu, Qisheng ; Han, Qianqian ; Zhang, Lan ; Sui, Lei ; Zheng, Leilei ; Meng, Shu ; Tang, Yin ; Xuan, Dongying ; Zhang, Jin ; Murray, Dana ; Shen, Qingping ; Cheng, Jessica ; Kim, Sung Hoon ; Dong, Lily Q ; Valverde, Paloma ; Cao, Xinming ; Chen, Jake. / Adiponectin regulates bone marrow mesenchymal stem cell niche through a unique signal transduction pathway : An approach for treating bone disease in diabetes. In: Stem Cells. 2015 ; Vol. 33, No. 1. pp. 240-252.
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