Adhesion of platelets to purified solid-phase von Willebrand factor: Effects of wall shear rate, ADP, thrombin and ristocetin

John D Olson, Anthony Zaleski, Deborah Herrmann, Patricia A. Flood

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

When platelets are stimulated with adenosine diphosphate (ADP), thrombin, or ristocetin, they bind soluble von Willebrand factor (vWF). In contrast, platelets adhere to solid-phase vWF without apparent stimulus. This work characterizes the adhesion of washed human platelets to highly purified solid-phase human vWF. VWF (iodine 125-labeled vWF) was demonstrated to bind in a quantifiable fashion to the internal surfaces of glass capillary tubes, saturating at a surface density of 3.0 ng/ml. The multimeric structure of bound vWF was the same as that of normal vWF. Platelets were washed, labeled with Indium 111, and resus-pended with washed red blood cells (RBCs) in balanced salt solution containing Co+ +, Mg+ +, and apyrase. The washed platelet RBC suspension was aspirated through capillary tubes to which vWF was adsorbed. Adhesion of platelets to adsorbed vWF was directly dependent on the surface density of vWF. Increasing wall shear rate (100 to 5000 sec-1) produced increasing platelet adhesion to maximum reached at 2500 sec-1. Platelets bound to the solid-phase vWF in an irreversible fashion, and, as demonstrated with scanning electron microscopy, they spread on the surface. When used to stimulate the platelets, ADP, thrombin, and ristocetin all increased the platelet adhesion to solid-phase vWF. ADP- and thrombin-stimulated reactions were inhibited by prior treatment of the platelets with 5′-p-fluorosulfonylbenzoyl adenosine. This inhibitor of ADP binding had no effect on the baseline platelet adhesion reaction (without ADP or thrombin). Adenosine in concentrations up to 1 mmol/L failed to inhibit adhesion. The data demonstrate that washed platelets adhere to solid-phase vWF without added agonists, that the reaction is dependent on surface density vWF and wall shear rate, that they bind irreversibly, and that they demonstrate surface spreading. In addition, these platelets can be stimulated to increase their adherence to vWF by using ADP, thrombin, and ristocetin.

Original languageEnglish (US)
Pages (from-to)6-18
Number of pages13
JournalThe Journal of Laboratory and Clinical Medicine
Volume114
Issue number1
StatePublished - 1989
Externally publishedYes

Fingerprint

Ristocetin
von Willebrand Factor
Platelets
Thrombin
Adenosine Diphosphate
Shear deformation
Blood Platelets
Adhesion
Capillary tubes
Blood
Erythrocytes
Apyrase
Indium

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Adhesion of platelets to purified solid-phase von Willebrand factor : Effects of wall shear rate, ADP, thrombin and ristocetin. / Olson, John D; Zaleski, Anthony; Herrmann, Deborah; Flood, Patricia A.

In: The Journal of Laboratory and Clinical Medicine, Vol. 114, No. 1, 1989, p. 6-18.

Research output: Contribution to journalArticle

Olson, John D ; Zaleski, Anthony ; Herrmann, Deborah ; Flood, Patricia A. / Adhesion of platelets to purified solid-phase von Willebrand factor : Effects of wall shear rate, ADP, thrombin and ristocetin. In: The Journal of Laboratory and Clinical Medicine. 1989 ; Vol. 114, No. 1. pp. 6-18.
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