Adenovirus-mediated retinoblastoma gene therapy suppresses spontaneous pituitary melanotroph tumors in Rb(+/-) mice

Daniel J. Riley, Alexander Yu Nikitin, Wen Hwa Lee

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The retinoblastoma gene (RB) is the prototypic tumor suppressor. Studies to date have demonstrated cancer suppression with tumor cells reconstituted with RB ex vivo and implanted into immunodeficient mice, as well as with germline transmission of a human RB transgene into tumor-prone Rb(+/-) mice. To mimic the therapy of cancer more closely, spontaneous pituitary melanotroph tumors arising in immunocompetent Rb(+/-) mice were treated with a recombinant adenovirus carrying RB cDNA. Intratumoral RB gene transfer decreased tumor cell proliferation, reestablished innervation by growth-regulatory dopaminergic neurons, inhibited the growth of tumors, and prolonged the life spans of treated animals.

Original languageEnglish (US)
Pages (from-to)1316-1321
Number of pages6
JournalNature Medicine
Volume2
Issue number12
DOIs
StatePublished - Dec 1996

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Melanotrophs
Retinoblastoma Genes
Gene therapy
Pituitary Neoplasms
Adenoviridae
Genetic Therapy
Tumors
Genes
Neoplasms
Gene transfer
Cell proliferation
Dopaminergic Neurons
Neurons
Growth
Transgenes
Animals
Complementary DNA
Cells
Cell Proliferation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Adenovirus-mediated retinoblastoma gene therapy suppresses spontaneous pituitary melanotroph tumors in Rb(+/-) mice. / Riley, Daniel J.; Nikitin, Alexander Yu; Lee, Wen Hwa.

In: Nature Medicine, Vol. 2, No. 12, 12.1996, p. 1316-1321.

Research output: Contribution to journalArticle

Riley, Daniel J. ; Nikitin, Alexander Yu ; Lee, Wen Hwa. / Adenovirus-mediated retinoblastoma gene therapy suppresses spontaneous pituitary melanotroph tumors in Rb(+/-) mice. In: Nature Medicine. 1996 ; Vol. 2, No. 12. pp. 1316-1321.
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