Adenovirus E1A gene autorepression: Revertants of an E1A promoter mutation encode altered E1A proteins

P. L. Larsen, C. Tibbetts

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Revertants have been isolated from Ad3hr15, a mutant of human adenovirus type 3 that carries a defective E1A promoter. Transcription of these revertant E1A genes is restored - from nil for Ad3hr15 mutant to levels exceeding that of the wild-type virus. The mutant Ad3hr15 virus and the revertants all have an aberrant E1A promoter that contains two short tandem duplications of viral DNA sequence. The E1A gene-coding region of the mutant is the same as that for wild-type adenovirus type 3, whereas the revertants are characterized by short in-frame deletions within the 5' exon region of their E1A genes. Location of these reverting, second-site deletions is discussed in relation to E1A gene autoregulation and the evolved diversity of E1A gene autoregulation and the evolved diversity of E1A-related oncogenic potential among different human adenoviruses.

Original languageEnglish (US)
Pages (from-to)8185-8189
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number23
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • General

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