Adenosine effects on the rat pineal gland in vitro: Cyclic adenosine monophosphate levels, N‐acetyltransferase, and thyroxine type II 5′‐deiodinase activities and melatonin production

K. O. Nonaka, R. J. Reiter, B. Withyachumnarnkul, K. ‐A Stokkan, A. Lerchl

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Abstract: The presence of adenosine and its receptors in the pineal gland suggests that they could have a regulatory influence on pineal physiology. Rat pineal glands cultured in the presence of the adenosine analog 5′‐N‐ethylcarboxamido adenosine (NECA) promoted a significant increase in pineal adenosine 3′5′‐monophosphate (cAMP) content, similar to that caused by the ß‐adrenergic receptor agonist isoproterenol (ISO). A potentiation of the cAMP response occurred when the glands were simultaneously stimulated with both NECA and ISO. Whereas high cAMP levels induced by ISO were associated with an increased N‐acetyltransferase (NAT) activity and rise in the melatonin production and release, the NECA‐induced rise in cAMP concentration failed to promote an increase in the activity of either NAT or another pineal enzyme thyroxine 5′‐deiodinase type II; likewise, pineal melatonin levels did not rise.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of pineal research
Volume11
Issue number1
DOIs
StatePublished - Aug 1991

Keywords

  • 5′‐N‐ethylcarboxamido adenosine (NECA)
  • adenosine 3′5′‐monophosphate (cAMP)
  • adenosine type 2 receptor
  • pineal gland
  • thyroxine type II 5′‐deiodinase

ASJC Scopus subject areas

  • Endocrinology

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