Adduction of DNA with MTBE and TBA in mice studied by accelerator mass spectrometry

Y. Yuan; H. F. Wang; H. F. Sun; H. F. Du; L. H. Xu; Y. F. Liu; X. F. Ding; D. P. Fu; K. X. Liu

doi:10.1002/tox.20295

Adduction of DNA with MTBE and TBA in mice studied by accelerator mass spectrometry

Y. Yuan, H. F. Wang, H. F. Sun, H. F. Du, L. H. Xu, Y. F. Liu, X. F. Ding, D. P. Fu, K. X. Liu

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Methyl tert-butyl ether (MTBE) is a currently worldwide used octane enhancer substituting for lead alkyls and gasoline oxygenate. Our previous study using doubly ¹⁴C-labeled MTBE [(CH₃)₃ ¹⁴CO¹⁴CH₃] has shown that MTBE binds DNA to form DNA adducts at low dose levels in mice. To elucidate the mechanism of the binding reaction, in this study, the DNA adducts with singly ¹⁴C-labeled MTBE, which was synthesized from ¹⁴C-methanol and tert-butyl alcohol (TBA), or ¹⁴C-labeled TBA in mice have been measured by ultra sensitive accelerator mass spectrometry. The results show that the methyl group of MTBE and tert-butyl alcohol definitely form adducts with DNA in mouse liver, lung, and kidney. The methyl group of MTBE is the predominant binding part in liver, while the methyl group and the tert-butyl group give comparable contributions to the adduct formation in lung and kidney.

Original language	English (US)
Pages (from-to)	630-635
Number of pages	6
Journal	Environmental Toxicology
Volume	22
Issue number	6
DOIs	https://doi.org/10.1002/tox.20295
State	Published - 2007
Externally published	Yes

Keywords

Accelerator mass spectrometry (AMS)
DNA adduct
Methyl tert-butyl ether (MTBE)
Tert-butyl alcohol (TBA)

ASJC Scopus subject areas

Health, Toxicology and Mutagenesis
Management, Monitoring, Policy and Law
Toxicology

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title = "Adduction of DNA with MTBE and TBA in mice studied by accelerator mass spectrometry",

abstract = "Methyl tert-butyl ether (MTBE) is a currently worldwide used octane enhancer substituting for lead alkyls and gasoline oxygenate. Our previous study using doubly 14C-labeled MTBE [(CH3)3 14CO14CH3] has shown that MTBE binds DNA to form DNA adducts at low dose levels in mice. To elucidate the mechanism of the binding reaction, in this study, the DNA adducts with singly 14C-labeled MTBE, which was synthesized from 14C-methanol and tert-butyl alcohol (TBA), or 14C-labeled TBA in mice have been measured by ultra sensitive accelerator mass spectrometry. The results show that the methyl group of MTBE and tert-butyl alcohol definitely form adducts with DNA in mouse liver, lung, and kidney. The methyl group of MTBE is the predominant binding part in liver, while the methyl group and the tert-butyl group give comparable contributions to the adduct formation in lung and kidney.",

keywords = "Accelerator mass spectrometry (AMS), DNA adduct, Methyl tert-butyl ether (MTBE), Tert-butyl alcohol (TBA)",

author = "Y. Yuan and Wang, {H. F.} and Sun, {H. F.} and Du, {H. F.} and Xu, {L. H.} and Liu, {Y. F.} and Ding, {X. F.} and Fu, {D. P.} and Liu, {K. X.}",

year = "2007",

doi = "10.1002/tox.20295",

language = "English (US)",

volume = "22",

pages = "630--635",

journal = "Environmental Toxicology",

issn = "1520-4081",

publisher = "John Wiley & Sons Inc.",

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T1 - Adduction of DNA with MTBE and TBA in mice studied by accelerator mass spectrometry

AU - Yuan, Y.

AU - Wang, H. F.

AU - Sun, H. F.

AU - Du, H. F.

AU - Xu, L. H.

AU - Liu, Y. F.

AU - Ding, X. F.

AU - Fu, D. P.

AU - Liu, K. X.

PY - 2007

Y1 - 2007

N2 - Methyl tert-butyl ether (MTBE) is a currently worldwide used octane enhancer substituting for lead alkyls and gasoline oxygenate. Our previous study using doubly 14C-labeled MTBE [(CH3)3 14CO14CH3] has shown that MTBE binds DNA to form DNA adducts at low dose levels in mice. To elucidate the mechanism of the binding reaction, in this study, the DNA adducts with singly 14C-labeled MTBE, which was synthesized from 14C-methanol and tert-butyl alcohol (TBA), or 14C-labeled TBA in mice have been measured by ultra sensitive accelerator mass spectrometry. The results show that the methyl group of MTBE and tert-butyl alcohol definitely form adducts with DNA in mouse liver, lung, and kidney. The methyl group of MTBE is the predominant binding part in liver, while the methyl group and the tert-butyl group give comparable contributions to the adduct formation in lung and kidney.

AB - Methyl tert-butyl ether (MTBE) is a currently worldwide used octane enhancer substituting for lead alkyls and gasoline oxygenate. Our previous study using doubly 14C-labeled MTBE [(CH3)3 14CO14CH3] has shown that MTBE binds DNA to form DNA adducts at low dose levels in mice. To elucidate the mechanism of the binding reaction, in this study, the DNA adducts with singly 14C-labeled MTBE, which was synthesized from 14C-methanol and tert-butyl alcohol (TBA), or 14C-labeled TBA in mice have been measured by ultra sensitive accelerator mass spectrometry. The results show that the methyl group of MTBE and tert-butyl alcohol definitely form adducts with DNA in mouse liver, lung, and kidney. The methyl group of MTBE is the predominant binding part in liver, while the methyl group and the tert-butyl group give comparable contributions to the adduct formation in lung and kidney.

KW - Accelerator mass spectrometry (AMS)

KW - DNA adduct

KW - Methyl tert-butyl ether (MTBE)

KW - Tert-butyl alcohol (TBA)

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JO - Environmental Toxicology

JF - Environmental Toxicology

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Adduction of DNA with MTBE and TBA in mice studied by accelerator mass spectrometry

Abstract

Keywords

ASJC Scopus subject areas

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