Abstract
Simian-human immunodeficiency virus (SHIV) infection in rhesus macaques (RMs) resembles human immunodeficiency virus type 1 (HIV-1) infection in humans and serves as a tool to evaluate candidate AIDS vaccines. HIV-1 clade A (HIV-A) predominates in parts of Africa. We constructed an R5 clade A SHIV (SHIV-A; strain SHIV-KNH1144) carrying env from a Kenyan HIV-A. SHIV-A underwent rapid serial passage through six RMs. To allow unbridled replication without adaptive immunity, we simultaneously ablated CD8 + and B cells with cytotoxic monoclonal antibodies in the next RM, resulting in extremely high viremia and CD4 + T-cell loss. Infected blood was then transferred into two non-immune-depleted RMs, where progeny SHIV-A showed increased replicative capacity and caused AIDS. We reisolated SHIV-KNH1144p4, which was replication competent in peripheral blood mononuclear cells (PBMC) of all RMs tested. Next-generation sequencing of early- and late-passage SHIV-A strains identified mutations that arose due to “fitness” virus optimization in the former and mutations exhibiting signatures typical for adaptive host immunity in the latter. “Fitness” mutations are best described as mutations that allow for better fit of the HIV-A Env with SIV-derived virion building blocks or host proteins and mutations in noncoding regions that accelerate virus replication, all of which result in the outgrowth of virus variants in the absence of adaptive T-cell and antibody-mediated host immunity. IMPORTANCE In this study, we constructed a simian-human immunodeficiency virus carrying an R5 Kenyan HIV-1 clade A env (SHIV-A). To bypass host immunity, SHIV-A was rapidly passaged in naive macaques or animals depleted of both CD8 + and B cells. Next-generation sequencing identified different mutations that resulted from optimization of viral replicative fitness either in the absence of adaptive immunity or due to pressure from adaptive immune responses.
| Original language | English (US) |
|---|---|
| Article number | e02267-18 |
| Journal | Journal of Virology |
| Volume | 93 |
| Issue number | 9 |
| DOIs | |
| State | Published - May 1 2019 |
Fingerprint
ASJC Scopus subject areas
- Microbiology
- Immunology
- Insect Science
- Virology
Cite this
Adaptation of an R5 simian-human immunodeficiency virus encoding an HIV clade a envelope with or without ablation of adaptive host immunity : Differential selection of viral mutants. / Zhou, Mingkui; Humbert, Michael; Mukhtar, Muhammad M.; Scinto, Hanna B.; Vyas, Hemant K.; Lakhashe, Samir K.; Byrareddy, Siddappa N.; Maurer, Gregor; Thorat, Swati; Owuor, Joshua; Lai, Zhao; Chen, Yidong; Griffiths, Anthony; Chenine, Agnès Laurence; Gumber, Sanjeev; Villinger, François; Montefiori, David; Ruprecht, Ruth M.
In: Journal of Virology, Vol. 93, No. 9, e02267-18, 01.05.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Adaptation of an R5 simian-human immunodeficiency virus encoding an HIV clade a envelope with or without ablation of adaptive host immunity
T2 - Differential selection of viral mutants
AU - Zhou, Mingkui
AU - Humbert, Michael
AU - Mukhtar, Muhammad M.
AU - Scinto, Hanna B.
AU - Vyas, Hemant K.
AU - Lakhashe, Samir K.
AU - Byrareddy, Siddappa N.
AU - Maurer, Gregor
AU - Thorat, Swati
AU - Owuor, Joshua
AU - Lai, Zhao
AU - Chen, Yidong
AU - Griffiths, Anthony
AU - Chenine, Agnès Laurence
AU - Gumber, Sanjeev
AU - Villinger, François
AU - Montefiori, David
AU - Ruprecht, Ruth M.
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Simian-human immunodeficiency virus (SHIV) infection in rhesus macaques (RMs) resembles human immunodeficiency virus type 1 (HIV-1) infection in humans and serves as a tool to evaluate candidate AIDS vaccines. HIV-1 clade A (HIV-A) predominates in parts of Africa. We constructed an R5 clade A SHIV (SHIV-A; strain SHIV-KNH1144) carrying env from a Kenyan HIV-A. SHIV-A underwent rapid serial passage through six RMs. To allow unbridled replication without adaptive immunity, we simultaneously ablated CD8 + and B cells with cytotoxic monoclonal antibodies in the next RM, resulting in extremely high viremia and CD4 + T-cell loss. Infected blood was then transferred into two non-immune-depleted RMs, where progeny SHIV-A showed increased replicative capacity and caused AIDS. We reisolated SHIV-KNH1144p4, which was replication competent in peripheral blood mononuclear cells (PBMC) of all RMs tested. Next-generation sequencing of early- and late-passage SHIV-A strains identified mutations that arose due to “fitness” virus optimization in the former and mutations exhibiting signatures typical for adaptive host immunity in the latter. “Fitness” mutations are best described as mutations that allow for better fit of the HIV-A Env with SIV-derived virion building blocks or host proteins and mutations in noncoding regions that accelerate virus replication, all of which result in the outgrowth of virus variants in the absence of adaptive T-cell and antibody-mediated host immunity. IMPORTANCE In this study, we constructed a simian-human immunodeficiency virus carrying an R5 Kenyan HIV-1 clade A env (SHIV-A). To bypass host immunity, SHIV-A was rapidly passaged in naive macaques or animals depleted of both CD8 + and B cells. Next-generation sequencing identified different mutations that resulted from optimization of viral replicative fitness either in the absence of adaptive immunity or due to pressure from adaptive immune responses.
AB - Simian-human immunodeficiency virus (SHIV) infection in rhesus macaques (RMs) resembles human immunodeficiency virus type 1 (HIV-1) infection in humans and serves as a tool to evaluate candidate AIDS vaccines. HIV-1 clade A (HIV-A) predominates in parts of Africa. We constructed an R5 clade A SHIV (SHIV-A; strain SHIV-KNH1144) carrying env from a Kenyan HIV-A. SHIV-A underwent rapid serial passage through six RMs. To allow unbridled replication without adaptive immunity, we simultaneously ablated CD8 + and B cells with cytotoxic monoclonal antibodies in the next RM, resulting in extremely high viremia and CD4 + T-cell loss. Infected blood was then transferred into two non-immune-depleted RMs, where progeny SHIV-A showed increased replicative capacity and caused AIDS. We reisolated SHIV-KNH1144p4, which was replication competent in peripheral blood mononuclear cells (PBMC) of all RMs tested. Next-generation sequencing of early- and late-passage SHIV-A strains identified mutations that arose due to “fitness” virus optimization in the former and mutations exhibiting signatures typical for adaptive host immunity in the latter. “Fitness” mutations are best described as mutations that allow for better fit of the HIV-A Env with SIV-derived virion building blocks or host proteins and mutations in noncoding regions that accelerate virus replication, all of which result in the outgrowth of virus variants in the absence of adaptive T-cell and antibody-mediated host immunity. IMPORTANCE In this study, we constructed a simian-human immunodeficiency virus carrying an R5 Kenyan HIV-1 clade A env (SHIV-A). To bypass host immunity, SHIV-A was rapidly passaged in naive macaques or animals depleted of both CD8 + and B cells. Next-generation sequencing identified different mutations that resulted from optimization of viral replicative fitness either in the absence of adaptive immunity or due to pressure from adaptive immune responses.
UR - http://www.scopus.com/inward/record.url?scp=85065022244&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85065022244&partnerID=8YFLogxK
U2 - 10.1128/JVI.02267-18
DO - 10.1128/JVI.02267-18
M3 - Article
C2 - 30760566
AN - SCOPUS:85065022244
VL - 93
JO - Journal of Virology
JF - Journal of Virology
SN - 0022-538X
IS - 9
M1 - e02267-18
ER -