ADAM17 mediates Nox4 expression and NADPH oxidase activity in the kidney cortex of OVE26 mice

Bridget M. Ford, Assaad A. Eid, Monika Göoz, Jeffrey L. Barnes, Yves C. Gorin, Hanna E. Abboud

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Matrix protein accumulation is a prominent feature of diabetic nephropathy that contributes to renal fibrosis and decline in renal function. The pathogenic mechanisms of matrix accumulation are incompletely characterized. We investigated if the matrix metalloprotease a disintegrin and metalloprotease1 7 (ADAM17), known to cleave growth factors and cytokines, is activated in the kidney cortex of OVE26 type 1 diabetic mice and the potential mechanisms by which ADAM17 mediates extracellular matrix accumulation. Protein expression and activity of ADAM17 were increased in OVE26 kidney cortex. Using a pharmacological inhibitor to ADAM17, TMI-005, we determined that ADAM17 activation results in increased type IV collagen, Nox4, and NADPH oxidase activity in the kidney cortex of diabetic mice. In cultured mouse proximal tubular epithelial cells (MCTs), high glucose increases ADAM17 activity, Nox4 and fibronectin expression, cellular collagen content, and NADPH oxidase activity. These effects of glucose were inhibited when cells were pretreated with TMI-005 and/or transfected with small interfering ADAM17. Collectively, these data indicate a novel mechanism whereby hyperglycemia in diabetes increases extracellular matrix protein expression in the kidney cortex through activation of ADAM17 and enhanced oxidative stress through Nox enzyme activation. Additionally, our study is the first to provide evidence that Nox4 is downstream of ADAM17.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume305
Issue number3
DOIs
StatePublished - Aug 1 2013

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Disintegrins
Kidney Cortex
NADPH Oxidase
Kidney
Glucose
Enzyme Activation
Collagen Type IV
Extracellular Matrix Proteins
Diabetic Nephropathies
Metalloproteases
Fibronectins
Hyperglycemia
Extracellular Matrix
Intercellular Signaling Peptides and Proteins
Proteins
Oxidative Stress
Fibrosis
Collagen
Epithelial Cells
Pharmacology

Keywords

  • ADAM17
  • Diabetic nephropathy
  • Extracellular matrix
  • Nox4
  • OVE26 mice

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

ADAM17 mediates Nox4 expression and NADPH oxidase activity in the kidney cortex of OVE26 mice. / Ford, Bridget M.; Eid, Assaad A.; Göoz, Monika; Barnes, Jeffrey L.; Gorin, Yves C.; Abboud, Hanna E.

In: American Journal of Physiology - Renal Physiology, Vol. 305, No. 3, 01.08.2013.

Research output: Contribution to journalArticle

Ford, Bridget M. ; Eid, Assaad A. ; Göoz, Monika ; Barnes, Jeffrey L. ; Gorin, Yves C. ; Abboud, Hanna E. / ADAM17 mediates Nox4 expression and NADPH oxidase activity in the kidney cortex of OVE26 mice. In: American Journal of Physiology - Renal Physiology. 2013 ; Vol. 305, No. 3.
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