Acute alcohol intoxication potentiates neutrophil-mediated intestinal tissue damage after burn injury

Xiaoling Li, Martin G Schwacha, Irshad H. Chaudry, Mashkoor A. Choudhry

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

This study examined whether acute alcohol (EtOH) intoxication before burn injury potentiates postburn intestinal tissue damage and whether neutrophils have any role in the damage under those conditions. Male rats (∼250 g) were gavaged with EtOH to achieve a blood EtOH level of approximately 100 mg/dL or with saline and received either approximately 12.5% or approximately 25% total body surface area (TBSA) burn or sham injury. Rats were killed at 4 or 24 h after injury, and various parameters were measured. As compared with sham animals, burn injury alone (regardless of size) resulted in a significant increase in intestinal tissue myeloperoxidase (MPO; an index of neutrophil infiltration) activity and IL-18 levels 4 h after injury. Furthermore, rats receiving 25% TBSA, but not 12.5%, burn exhibited intestine edema. The IL-18 and MPO activity were normalized at 24 h after injury in rats receiving 12.5% TBSA burn, whereas these parameters remained elevated at 24 h in rats with 25% burn. The presence of EtOH in rats at the time of burn injury exacerbated the levels of IL-18, MPO activity, and edema at 4 and 24 h after burn injury. Treatment of rats with anti-IL-18 antibodies or with antineutrophil antiserum prevented the increase in the above parameters after EtOH and burn injury, except that the depletion of neutrophils did not prevent the IL-18 increase. In summary, these findings suggest that acute EtOH intoxication exacerbates postburn intestinal tissue damage after burn injury, and that it is, in part, neutrophil mediated.

Original languageEnglish (US)
Pages (from-to)377-383
Number of pages7
JournalShock
Volume29
Issue number3
DOIs
StatePublished - Mar 2008
Externally publishedYes

Fingerprint

Alcoholic Intoxication
Neutrophils
Interleukin-18
Wounds and Injuries
Body Surface Area
Edema
Neutrophil Infiltration
Peroxidase
Intestines
Immune Sera

Keywords

  • Cytokines
  • Ethanol
  • Inflammation
  • Intestine permeability
  • Myeloperoxidase
  • Trauma

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine
  • Physiology

Cite this

Acute alcohol intoxication potentiates neutrophil-mediated intestinal tissue damage after burn injury. / Li, Xiaoling; Schwacha, Martin G; Chaudry, Irshad H.; Choudhry, Mashkoor A.

In: Shock, Vol. 29, No. 3, 03.2008, p. 377-383.

Research output: Contribution to journalArticle

Li, Xiaoling ; Schwacha, Martin G ; Chaudry, Irshad H. ; Choudhry, Mashkoor A. / Acute alcohol intoxication potentiates neutrophil-mediated intestinal tissue damage after burn injury. In: Shock. 2008 ; Vol. 29, No. 3. pp. 377-383.
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