Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/- mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays

Martin L. Meltz, James T. MacGregor

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

The activity of quercetin was investigated in (a) the L5178Y TK+/- mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/- mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.

Original languageEnglish (US)
Pages (from-to)317-324
Number of pages8
JournalMutation Research/Genetic Toxicology
Volume88
Issue number3
DOIs
StatePublished - Mar 1981

ASJC Scopus subject areas

  • Toxicology
  • Genetics

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