Activity of respiratory neurons during NREM sleep

J. Orem, I. Osorio, E. Brooks, T. Dick

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The purpose of this study was to analyze differences in the activity of medullary respiratory neurons in the unanesthetized, intact cat during wakefulness and non-rapid-eye-movement (NREM) sleep. We studied single respiratory neurons located within a 1-2 mm deep, 8-10 mm long zone that followed, and included in its dorsal aspect, the retrofacial and ambiguus nuclei. The analysis of variance was used to detect respiratory activity (7), and cycle-triggered histograms were plotted. The respiratory signal strength and consistency of the respiratory activity were quantified with the η2 statistic. We determined for each breath in wakefulness and NREM sleep the average discharge rate during the active phase of the cell, the number of action potentials during the active phase of the cell, and durations of both the cycle and inspiration. Differences in discharge rates and in the number of discharges between wakefulness and NREM sleep were tested with the t test. A bimodal distribution of η2 values for the population of neurons indicated there were two groups of respiratory cells: those with η2 values <0.3 and those with values >0.3. The former we call weak respiratory cells; the latter, strong respiratory cells. Strong and weak cells were classified further as inspiratory or non-inspiratory on the basis of the shape of their cycle-triggered histograms. Within the class of strong inspiratory cells, those with the highest η2 values 1) reached their peak discharge rate early, 2) discharged at high rates throughout inspiration, and 3) were inactive during expiration. The values of these variables diminished progressively in inspiratory cell groups with lower η2 values. Most cells were less active in NREM sleep than in wakefulness. Similar proportions of weak and strong cells and inspiratory and noninspiratory cells were affected by sleep. The reduction in sleep of the activity of strong inspiratory cells was consistent with a general relationship between this activity and the duration of inspiration. Lower discharge rates were associated with longer breaths; higher rates with shorter breaths. This relationship existed within both NREM sleep and wakefulness, and the plot of the relationship across these states formed a continuous function. The reduction in discharge rate in sleep was greater for weak than for strong inspiratory cells: the correlation coefficient between percent change in rate and η2 values was -0.636 for inspiratory cells, but it was not significant (-0.265) for noninspiratory cells. The relationship between change in rate and η2 value was best within the classes of strong cells: the correlation coefficient was -0.678 for strong inspiratory cells and -0.648 for strong noninspiratory cells. If we assume that the η2 value of activity is related inversely to the amount of nonrespiratory afference contributing to that activity, these results indicate that the effect of sleep is greater on cells receiving larger proportions of nonrespiratory afference.

Original languageEnglish (US)
Pages (from-to)1144-1156
Number of pages13
JournalJournal of neurophysiology
Volume54
Issue number5
DOIs
StatePublished - 1985
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

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