Activity detection of GalNAc transferases by protein-based fluorescence sensors in vivo

Lina Song, Collin Bachert, Adam D. Linstedt

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Mucin-type O-glycosylation occurring in the Golgi apparatus is an important protein posttranslational modification initiated by up to 20 GalNAc-transferase isozymes with largely distinct substrate specificities. Regulation of this enzyme family affects a vast array of proteins transiting the secretory pathway and misregulation causes human diseases. Here we describe the use of protein-based fluorescence sensors that traffic in the secretory pathway to monitor GalNAc-transferase activity in living cells. The sensors can either be “pan” or isozyme specific.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages123-131
Number of pages9
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1496
ISSN (Print)1064-3745

Keywords

  • Fluorescence-activating protein
  • Fluorescent biosensor
  • GalNAc transferase
  • O-glycosylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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