Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption

Jun Liu, Ming Gao, Michael Nipper, Janice Deng, Francis E Sharkey, Randy L. Johnson, Howard C. Crawford, Yidong Chen, Pei Wang

Research output: Contribution to journalArticle

Abstract

Damaged acinar cells play a passive role in activating pancreatic stellate cells (PSCs) via recruitment of immune cells that subsequently activate PSCs. However, whether acinar cells directly contribute to PSC activation is unknown. Here, we report that the Hippo pathway, a well-known regulator of proliferation, is essential for suppression of expression of inflammation and fibrosis-associated genes in adult pancreatic acinar cells. Hippo inactivation in acinar cells induced yes-associated protein 1 (YAP1)/transcriptional coactivator with PDZ binding motif (TAZ)-dependent, irreversible fibrosis and inflammation, which was initiated by Hippo-mediated acinar-stromal communications and ameliorated by blocking YAP1/TAZ target connective tissue growth factor (CTGF). Hippo disruption promotes acinar cells to secrete fibroinflammatory factors and induce stromal activation, which precedes acinar proliferation and metaplasia. We found that Hippo disruption did not induce cell-autonomous proliferation but primed acinar cells to exogenous pro-proliferative stimuli, implying a well-orchestrated scenario in which Hippo signaling acts as an intrinsic link to coordinate fibroinflammatory response and proliferation for maintenance of the tissue integrity. Our findings suggest that the fibroinflammatory program in pancreatic acinar cells is suppressed under normal physiological conditions. While transient activation of inflammatory gene expression during tissue injury may contribute to the control of damage and tissue repair, its persistent activation may result in tissue fibrosis and failure of regeneration.

Original languageEnglish (US)
Pages (from-to)e3000418
JournalPLoS biology
Volume17
Issue number9
DOIs
StatePublished - Sep 1 2019

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acinar cells
Acinar Cells
Chemical activation
Tissue
Pancreatic Stellate Cells
fibrosis
Fibrosis
Connective Tissue Growth Factor
Gene expression
inflammation
Proteins
cells
Repair
Inflammation
Genes
metaplasia
Metaplasia
tissue repair
Communication
connective tissues

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption. / Liu, Jun; Gao, Ming; Nipper, Michael; Deng, Janice; Sharkey, Francis E; Johnson, Randy L.; Crawford, Howard C.; Chen, Yidong; Wang, Pei.

In: PLoS biology, Vol. 17, No. 9, 01.09.2019, p. e3000418.

Research output: Contribution to journalArticle

Liu, J, Gao, M, Nipper, M, Deng, J, Sharkey, FE, Johnson, RL, Crawford, HC, Chen, Y & Wang, P 2019, 'Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption', PLoS biology, vol. 17, no. 9, pp. e3000418. https://doi.org/10.1371/journal.pbio.3000418
Liu, Jun ; Gao, Ming ; Nipper, Michael ; Deng, Janice ; Sharkey, Francis E ; Johnson, Randy L. ; Crawford, Howard C. ; Chen, Yidong ; Wang, Pei. / Activation of the intrinsic fibroinflammatory program in adult pancreatic acinar cells triggered by Hippo signaling disruption. In: PLoS biology. 2019 ; Vol. 17, No. 9. pp. e3000418.
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