Activation of liver mitochondrial phospholipase A2 by superoxide

M. Madesh, K. A. Balasubramanian

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Mitochondrial damage is one of the prominent features of cell injury during oxidative stress and altered mitochondrial lipids may contribute to this damage. Lipid changes were observed when liver mitochondria were exposed to superoxide generating systems. Phosphatidylcholine and phosphatidylethanolamine contents were decreased with simultaneous formation of lysophospholipids when exposed to superoxide. Among the neutral lipids there was an increase in the level of free fatty acids. This alteration in lipid composition could be prevented by the simultaneous presence of superoxide dismutase or phospholipase A2 (PLA2) inhibitors. H2O2 did not have any effect on liver mitochondrial PLA2. This suggests that superoxide anion stimulates phospholipase A2 which is prevented by superoxide scavenging agents and PLA2 inhibitors. The products of phospholipase A2 are membrane- damaging agents which may be responsible for mitochondrial damage seen during oxidative stress.

Original languageEnglish (US)
Pages (from-to)187-192
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume346
Issue number2
DOIs
StatePublished - Oct 15 1997
Externally publishedYes

Keywords

  • Liver mitochondria
  • Phospholipase A2
  • Superoxide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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