Activation of intestinal mitochondrial phospholipase D by polyamines and monoamines

Muniswamy Madesh; Kunissery A. Balasubramanian

doi:10.1016/S0005-2760(97)00074-X

Activation of intestinal mitochondrial phospholipase D by polyamines and monoamines

Muniswamy Madesh, Kunissery A. Balasubramanian

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Intestinal mitochondria have a phospholipase D (PLD) activity which was stimulated by polyamines and monoamines resulting in the formation of phosphatidic acid (PA) from endogenous phospholipids. When stimulated by polyamines, mitochondrial PLD utilized endogenous phosphatidylethanolamine (PE) as substrate whereas stimulated by monoamines, both PE and phosphatidylcholine (PC) were hydrolysed. Stimulation of PA formation by spermine was enhanced by the presence of calcium. Since polyamines are known to alter the calcium transport by mitochondria and PA is known to possess an ionophore effect, stimulation of PA formation in mitochondria by polyamines suggests that polyamine-induced alteration in calcium homeostasis might involve a PA related mechanism.

Original language	English (US)
Pages (from-to)	324-330
Number of pages	7
Journal	Biochimica et Biophysica Acta - Lipids and Lipid Metabolism
Volume	1348
Issue number	3
DOIs	https://doi.org/10.1016/S0005-2760(97)00074-X
State	Published - Oct 18 1997
Externally published	Yes

Keywords

Intestinal mitochondria
Monoamines
Phosphatidic acid
Phospholipase D
Polyamines

ASJC Scopus subject areas

Endocrinology
Biophysics
Biochemistry

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10.1016/S0005-2760(97)00074-X

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title = "Activation of intestinal mitochondrial phospholipase D by polyamines and monoamines",

abstract = "Intestinal mitochondria have a phospholipase D (PLD) activity which was stimulated by polyamines and monoamines resulting in the formation of phosphatidic acid (PA) from endogenous phospholipids. When stimulated by polyamines, mitochondrial PLD utilized endogenous phosphatidylethanolamine (PE) as substrate whereas stimulated by monoamines, both PE and phosphatidylcholine (PC) were hydrolysed. Stimulation of PA formation by spermine was enhanced by the presence of calcium. Since polyamines are known to alter the calcium transport by mitochondria and PA is known to possess an ionophore effect, stimulation of PA formation in mitochondria by polyamines suggests that polyamine-induced alteration in calcium homeostasis might involve a PA related mechanism.",

keywords = "Intestinal mitochondria, Monoamines, Phosphatidic acid, Phospholipase D, Polyamines",

author = "Muniswamy Madesh and Balasubramanian, {Kunissery A.}",

note = "Funding Information: The Wellcome Trust Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Department of Science and Technology and the Indian Council of Medical Research, Government of India is gratefully acknowledged. The authors thank Prof. V.I. Mathan for his keen interest in this work. M. Madesh is a Senior Research Fellow of the Council of Scientific and Industrial Research, India. ",

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doi = "10.1016/S0005-2760(97)00074-X",

language = "English (US)",

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TY - JOUR

T1 - Activation of intestinal mitochondrial phospholipase D by polyamines and monoamines

AU - Madesh, Muniswamy

AU - Balasubramanian, Kunissery A.

N1 - Funding Information: The Wellcome Trust Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Department of Science and Technology and the Indian Council of Medical Research, Government of India is gratefully acknowledged. The authors thank Prof. V.I. Mathan for his keen interest in this work. M. Madesh is a Senior Research Fellow of the Council of Scientific and Industrial Research, India.

PY - 1997/10/18

Y1 - 1997/10/18

N2 - Intestinal mitochondria have a phospholipase D (PLD) activity which was stimulated by polyamines and monoamines resulting in the formation of phosphatidic acid (PA) from endogenous phospholipids. When stimulated by polyamines, mitochondrial PLD utilized endogenous phosphatidylethanolamine (PE) as substrate whereas stimulated by monoamines, both PE and phosphatidylcholine (PC) were hydrolysed. Stimulation of PA formation by spermine was enhanced by the presence of calcium. Since polyamines are known to alter the calcium transport by mitochondria and PA is known to possess an ionophore effect, stimulation of PA formation in mitochondria by polyamines suggests that polyamine-induced alteration in calcium homeostasis might involve a PA related mechanism.

AB - Intestinal mitochondria have a phospholipase D (PLD) activity which was stimulated by polyamines and monoamines resulting in the formation of phosphatidic acid (PA) from endogenous phospholipids. When stimulated by polyamines, mitochondrial PLD utilized endogenous phosphatidylethanolamine (PE) as substrate whereas stimulated by monoamines, both PE and phosphatidylcholine (PC) were hydrolysed. Stimulation of PA formation by spermine was enhanced by the presence of calcium. Since polyamines are known to alter the calcium transport by mitochondria and PA is known to possess an ionophore effect, stimulation of PA formation in mitochondria by polyamines suggests that polyamine-induced alteration in calcium homeostasis might involve a PA related mechanism.

KW - Intestinal mitochondria

KW - Monoamines

KW - Phosphatidic acid

KW - Phospholipase D

KW - Polyamines

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JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

IS - 3

ER -

Activation of intestinal mitochondrial phospholipase D by polyamines and monoamines

Abstract

Keywords

ASJC Scopus subject areas

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