Activation of excitatory amino acid receptors in bovine dental pulp evokes the release of iCGRP

D. L. Jackson, Kenneth M Hargreaves

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The activation of excitatory amino acid (EAA) receptors within the central nervous system is associated with numerous centrally mediated phenomena, including hyperalgesia. However, relatively little is known about the peripheral mechanisms which these receptors may regulate when activated. This research evaluated the hypothesis that EAA receptors in bovine dental pulp activate a population of peptidergic sensory neurons as measured by the release of immunoreactive calcitonin gene-related peptide (iCGRP), a neuropeptide associated with neurogenic inflammation. In vitro superfusion of bovine dental pulp was used to evaluate the regulation of iCGRP secretion by the EAA receptor agonists AMPA, kainate, NMDA, and L-glutamate. Both AMPA and kainate stimulated the release of iCGRP in a concentration-dependent manner (AMPA EC50 = 0.27 ± 3.3 nM; kainate EC50 = 3.2 ± 1.1 μM). Pre-treatment and co-administration of the AMPA/kainate receptor antagonist CNQX significantly reduced the iCGRP release evoked by either of these agonists. In contrast, neither NMDA nor L-glutamate induced any consistent changes in iCGRP release. These results suggest that the activation of AMPA and kainate receptors in dental pulp may contribute to peripheral release of vasoactive neuropeptides which mediate a neurogenic component of inflammation.

Original languageEnglish (US)
Pages (from-to)54-60
Number of pages7
JournalJournal of Dental Research
Volume78
Issue number1
StatePublished - 1999
Externally publishedYes

Fingerprint

Aminoacylation
Dental Pulp
Calcitonin Gene-Related Peptide
Glutamate Receptors
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Kainic Acid
Neurogenic Inflammation
Kainic Acid Receptors
AMPA Receptors
N-Methylaspartate
Neuropeptides
Glutamic Acid
6-Cyano-7-nitroquinoxaline-2,3-dione
Excitatory Amino Acid Agonists
Hyperalgesia
Sensory Receptor Cells
Central Nervous System
Research
Population

Keywords

  • α-amino-3-hydroxy-5-methylisozazole-4-propionic acid (AMPA)
  • Calcitonin gene-related peptide (CGRP)
  • Dental pulp
  • Excitatory amino acids
  • Kainate

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Activation of excitatory amino acid receptors in bovine dental pulp evokes the release of iCGRP. / Jackson, D. L.; Hargreaves, Kenneth M.

In: Journal of Dental Research, Vol. 78, No. 1, 1999, p. 54-60.

Research output: Contribution to journalArticle

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