Activation of estrogen receptor-α by E2 or EGF induces temporally distinct patterns of large-scale chromatin modification and mRNA transcription

Valeria Berno, Larbi Amazit, Cruz Hinojos, Jeannie Zhong, Maureen G. Mancini, Zelton D Sharp, Michael A. Mancini

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Estrogen receptor-α (ER) transcription function is regulated in a ligand-dependent (e.g, estradiol, E2) or ligand-independent (e.g., growth factors) manner. Our laboratory seeks to understand these two modes of action. Using a cell line that contains a visible prolactin enhancer/promoter array (PRL-HeLa) regulated by ER, we analyzed ER response to E2 and EGF by quantifying image-based results. Data show differential recruitment of GFP-ER to the array, with the AF1 domain playing a vital role in EGF-mediated responsiveness. Temporal analyses of large-scale chromatin dynamics, and accumulation of array-localized reporter mRNA over 24 hours showed that the EGF response consists of a single pulse of reporter mRNA accumulation concomitant with transient increase in array decondensation. Estradiol induced a novel cyclical pattern of mRNA accumulation with a sustained increase in array decondensation. Collectively, our work shows that there is a stimuli-specific pattern of large-scale chromatin modification and transcript levels by ER.

Original languageEnglish (US)
Article numbere2286
JournalPLoS One
Volume3
Issue number5
DOIs
StatePublished - May 28 2008

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Transcription
Epidermal Growth Factor
Estrogen Receptors
Chromatin
chromatin
transcription (genetics)
Chemical activation
Messenger RNA
estradiol
Estradiol
Ligands
prolactin
Prolactin
growth factors
mechanism of action
Intercellular Signaling Peptides and Proteins
Cells
promoter regions
cell lines
estrogen receptors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Activation of estrogen receptor-α by E2 or EGF induces temporally distinct patterns of large-scale chromatin modification and mRNA transcription. / Berno, Valeria; Amazit, Larbi; Hinojos, Cruz; Zhong, Jeannie; Mancini, Maureen G.; Sharp, Zelton D; Mancini, Michael A.

In: PLoS One, Vol. 3, No. 5, e2286, 28.05.2008.

Research output: Contribution to journalArticle

Berno, Valeria ; Amazit, Larbi ; Hinojos, Cruz ; Zhong, Jeannie ; Mancini, Maureen G. ; Sharp, Zelton D ; Mancini, Michael A. / Activation of estrogen receptor-α by E2 or EGF induces temporally distinct patterns of large-scale chromatin modification and mRNA transcription. In: PLoS One. 2008 ; Vol. 3, No. 5.
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AU - Zhong, Jeannie

AU - Mancini, Maureen G.

AU - Sharp, Zelton D

AU - Mancini, Michael A.

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