The role of cytosol components in the loss of rat liver adenylate cyclase activity which occurs during the preparation in particulate fractions from crude homogenates was studied. Epinephrine (5 μM)-, (10 μM)-, and fluoride (5 mM)- stimulated activities of twice-washed particulates were 31%, 58% and 67% of the homogenate activities, respectively. Addition of cytosol (100,000 x g supernatant devoid of adenylate cyclase activity restored these activities to 82%, 88% and 80%. Cytosol also increased particular basal activity from 60% of homogenate activity to 98%.
|Original language||English (US)|
|Number of pages||19|
|Journal||Journal of Cyclic Nucleotide Research|
|State||Published - Dec 1 1978|
ASJC Scopus subject areas