Activation by methylene blue of large Ca2+-activated K+ channels

James D. Stockand; Steven C. Sansom

doi:10.1016/S0005-2736(96)00194-0

Activation by methylene blue of large Ca²⁺-activated K⁺ channels

James D. Stockand
, Steven C. Sansom

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Using the patch-clamp method, we found that methylene blue (MB), a free radical inhibitor of guanylyl cyclase, activated large, Ca²⁺-activated K⁺ channels (BK(Ca)) in either cell attached or excised, inside-out patches of human mesangial cells in culture. Since BK(Ca) are important feedback regulators of contraction of smooth muscle and mesangial cells, these results indicate that MB may be an important opener of BK(Ca) channels and a regulator of vascular volume and resistance.

Original language	English (US)
Pages (from-to)	123-126
Number of pages	4
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1285
Issue number	2
DOIs	https://doi.org/10.1016/S0005-2736(96)00194-0
State	Published - Dec 4 1996
Externally published	Yes

Keywords

Methylene blue
Potassium ion

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/S0005-2736(96)00194-0

Cite this

@article{25ee11a1a55642569dfeee3c337d6bb0,

title = "Activation by methylene blue of large Ca2+-activated K+ channels",

abstract = "Using the patch-clamp method, we found that methylene blue (MB), a free radical inhibitor of guanylyl cyclase, activated large, Ca2+-activated K+ channels (BK(Ca)) in either cell attached or excised, inside-out patches of human mesangial cells in culture. Since BK(Ca) are important feedback regulators of contraction of smooth muscle and mesangial cells, these results indicate that MB may be an important opener of BK(Ca) channels and a regulator of vascular volume and resistance.",

keywords = "Methylene blue, Potassium ion",

author = "Stockand, \{James D.\} and Sansom, \{Steven C.\}",

note = "Funding Information: In conclusion, our results can explain some of the paradoxical and ambiguous effects of methylene blue on the response of vascular cells to relaxing agents. Based on these results, LY83583 would be a more suitable agent for determining the signaling pathways for relaxing agents. The notion that methylene blue can open BKc, ` channels could have therapeutic consequences for regulating glomerular filtration rate or vascular smooth muscle tension, The human glomerular mesangial cells were a generous gift fi'um Hanna Abboud (U of TX-HSC. San Antonio). Steve Sansom was supported by American Diabetes Association award \#89,",

year = "1996",

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doi = "10.1016/S0005-2736(96)00194-0",

language = "English (US)",

volume = "1285",

pages = "123--126",

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T1 - Activation by methylene blue of large Ca2+-activated K+ channels

AU - Stockand, James D.

AU - Sansom, Steven C.

N1 - Funding Information: In conclusion, our results can explain some of the paradoxical and ambiguous effects of methylene blue on the response of vascular cells to relaxing agents. Based on these results, LY83583 would be a more suitable agent for determining the signaling pathways for relaxing agents. The notion that methylene blue can open BKc, ` channels could have therapeutic consequences for regulating glomerular filtration rate or vascular smooth muscle tension, The human glomerular mesangial cells were a generous gift fi'um Hanna Abboud (U of TX-HSC. San Antonio). Steve Sansom was supported by American Diabetes Association award #89,

PY - 1996/12/4

Y1 - 1996/12/4

N2 - Using the patch-clamp method, we found that methylene blue (MB), a free radical inhibitor of guanylyl cyclase, activated large, Ca2+-activated K+ channels (BK(Ca)) in either cell attached or excised, inside-out patches of human mesangial cells in culture. Since BK(Ca) are important feedback regulators of contraction of smooth muscle and mesangial cells, these results indicate that MB may be an important opener of BK(Ca) channels and a regulator of vascular volume and resistance.

AB - Using the patch-clamp method, we found that methylene blue (MB), a free radical inhibitor of guanylyl cyclase, activated large, Ca2+-activated K+ channels (BK(Ca)) in either cell attached or excised, inside-out patches of human mesangial cells in culture. Since BK(Ca) are important feedback regulators of contraction of smooth muscle and mesangial cells, these results indicate that MB may be an important opener of BK(Ca) channels and a regulator of vascular volume and resistance.

KW - Methylene blue

KW - Potassium ion

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