Actin cytoskeletons regulate the stretch-induced increase of Ca2+ current in human gastric myocytes

Jun Hee Kim, Poong Lyul Rhee, Tong Mook Kang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Using the whole-cell and single channel recording techniques, the influence of actin cytoskeletons on L-type Ca2+ current was investigated in human gastric smooth muscle cells. In isotonic condition, an actin depolymerizer cytochalasin D (Cyt-D) markedly decreased the whole-cell current (IBa) without changing steady-state voltage dependency and single channel conductance. Intracellular dialysis of phalloidin, an actin polymerizer, significantly increased the IBa. Hypotonic stretch (222 mOsm/L) of the myocytes increased the IBa, and Cyt-D significantly inhibited the IBa increase by the stretch. Phalloidin was without effect on the IBa increase by the stretch. Phalloidin antagonized the Cyt-D inhibition of the stretch-induced IBa increase. Neither heterotrimeric G protein modifiers (GTPγS and GDPβS) nor rho GTPase inhibitor (C3 exoenzyme) influenced the stretch-induced responses. These results reveal that the integrity of the actin cytoskeleton is an important factor which determines the activity of L-type Ca2+ channels and a response to stretch.

Original languageEnglish (US)
Pages (from-to)503-508
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume352
Issue number2
DOIs
StatePublished - Jan 12 2007
Externally publishedYes

Keywords

  • Actin cytoskeletons
  • Cytochalasin D
  • Human stomach
  • L-type Ca channels
  • Phalloidin
  • Smooth muscle
  • Stretch
  • Swelling

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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