Acetylcholine receptor-reactive antibody induces nitric oxide production by a rat skeletal muscle cell line: Influence of cytokine environment

Yvonne R. Garcia, Jennifer J. May, Alicia M. Green, Keith A. Krolick

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The monoclonal Lewis rat skeletal muscle cell line, LE1, responded to the acetylcholine receptor (AChR)-reactive antibody mAb35 by up-regulating levels of mRNA for inducible nitric oxide synthase (iNOS/NOS-II), followed by levels of NO. Interferon-gamma (IFN-γ) and interleukin-1 (IL-1) were also each capable of inducing iNOS message, and synergistically with mAb35. Finally, myocyte-derived NO was implicated as a possible source of immunomodulation in experimental autoimmune myasthenia gravis (EAMG), as shown by the ability of the culture fluids from IFN-γ-activated LE1 cells to inhibit the proliferation of AChR-reactive T cells.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalJournal of Neuroimmunology
Volume120
Issue number1-2
DOIs
StatePublished - Nov 10 2001

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Keywords

  • Autoimmune disease
  • Experimental autoimmune myasthenia gravis
  • Inflammatory cytokines
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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