TY - JOUR
T1 - Acetylcholine receptor-reactive antibody induces nitric oxide production by a rat skeletal muscle cell line
T2 - Influence of cytokine environment
AU - Garcia, Yvonne R.
AU - May, Jennifer J.
AU - Green, Alicia M.
AU - Krolick, Keith A.
N1 - Funding Information:
This research was supported by a grant from the National Institutes of Health (R01 NS/AI 36313, NS38904 and NS42116). We express our thanks to Irma Gonzales for her technical support, and to Dr. Y. Mulhern for many helpful discussions.
PY - 2001
Y1 - 2001
N2 - The monoclonal Lewis rat skeletal muscle cell line, LE1, responded to the acetylcholine receptor (AChR)-reactive antibody mAb35 by up-regulating levels of mRNA for inducible nitric oxide synthase (iNOS/NOS-II), followed by levels of NO. Interferon-gamma (IFN-γ) and interleukin-1 (IL-1) were also each capable of inducing iNOS message, and synergistically with mAb35. Finally, myocyte-derived NO was implicated as a possible source of immunomodulation in experimental autoimmune myasthenia gravis (EAMG), as shown by the ability of the culture fluids from IFN-γ-activated LE1 cells to inhibit the proliferation of AChR-reactive T cells.
AB - The monoclonal Lewis rat skeletal muscle cell line, LE1, responded to the acetylcholine receptor (AChR)-reactive antibody mAb35 by up-regulating levels of mRNA for inducible nitric oxide synthase (iNOS/NOS-II), followed by levels of NO. Interferon-gamma (IFN-γ) and interleukin-1 (IL-1) were also each capable of inducing iNOS message, and synergistically with mAb35. Finally, myocyte-derived NO was implicated as a possible source of immunomodulation in experimental autoimmune myasthenia gravis (EAMG), as shown by the ability of the culture fluids from IFN-γ-activated LE1 cells to inhibit the proliferation of AChR-reactive T cells.
KW - Autoimmune disease
KW - Experimental autoimmune myasthenia gravis
KW - Inflammatory cytokines
KW - Nitric oxide synthase
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U2 - 10.1016/S0165-5728(01)00414-3
DO - 10.1016/S0165-5728(01)00414-3
M3 - Article
C2 - 11694325
AN - SCOPUS:0034750868
VL - 120
SP - 103
EP - 111
JO - Advances in Neuroimmunology
JF - Advances in Neuroimmunology
SN - 0165-5728
IS - 1-2
ER -