Abstract
Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met.
Original language | English (US) |
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Pages (from-to) | 21-40 |
Number of pages | 20 |
Journal | Metabolites |
Volume | 1 |
Issue number | 1 |
DOIs | |
State | Published - Nov 11 2011 |
Externally published | Yes |
Keywords
- Electrospray ionization mass spectrometry
- Lipidomics
- Quantification
- Shotgun lipidomics
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry
- Molecular Biology