Accurate quantification of lipid species by electrospray ionization mass spectrometry - Meets a key challenge in lipidomics

Kui Yang, Xianlin Han

Research output: Contribution to journalReview articlepeer-review

102 Scopus citations

Abstract

Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met.

Original languageEnglish (US)
Pages (from-to)21-40
Number of pages20
JournalMetabolites
Volume1
Issue number1
DOIs
StatePublished - Nov 11 2011
Externally publishedYes

Keywords

  • Electrospray ionization mass spectrometry
  • Lipidomics
  • Quantification
  • Shotgun lipidomics

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology

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