Accumulation of α2u-globulin in the renal proximal tubules of male rats exposed to unleaded gasoline

Michael J. Olson, Bhagwan D. Garg, C. V. Ramana Murty, Arun K. Roy

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Saturated branched-chain aliphatic hydrocarbons, found in motor fuels, induce nephrotoxicity in male rats. Treatment of male rats with unleaded gasoline (0.04-2.0 ml/kg body wt, po) for 9 days increased markedly the number and size of hyaline (protein resorption) droplets in epithelial cells of the renal proximal convoluted tubules (PCT) and enhanced cellular exfoliation at high dose levels. No other treatment-related pathological effects were observed in the plomeruli, distal tubules, or medulla. The renal content of α2u-globulin, a major urinary protein of male rats, was increased maximally by about 4.4-fold after gasoline administration (1.0 ml/kg, po, 9 days); no further increase was observed at higher doses. Immunoperoxidase staining of kidney tissue sections for α2u-globulin revealed large accumulations of antigen localized in many of the PCT epithelial cells which contained hyaline droplets. The hepatic content of α2u-globulin and its mRNA were not altered by gasoline administration. These data show, for the first time, that α2u-globulin is accumulated in the kidneys of gasoline-intoxicated male rats and sequestered specifically in some of the hyaline droplets characteristic of gasoline-induced nephropathy. A hydrocarbon-induced defect in the renal lysosomal degradation of low-molecular-weight urinary proteins, rather than increased synthesis of these proteins, appears to cause hyaline droplet accumulation.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalToxicology and Applied Pharmacology
Volume90
Issue number1
DOIs
StatePublished - Aug 1987
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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