Absence of SHIV infection in gut and lymph node tissues in rhesus monkeys after repeated rectal challenges following HIV-1 DNA/MVA immunizations

Michael Aidoo, Ronald A. Otten, Vanessa Rodriguez, Carlos A. Sariol, Melween Martinez, Edmundo Kraiselburd, Harriet Robinson, Thomas Folks, Salvatore Butera, Dennis Ellenberger

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

We reported previously the absence of systemic infection in a subset of macaques vaccinated with an HIV-1 DNA/MVA vaccine after 18 or more rectal challenges with low (physiologically relevant) doses of SHIV162P3. To further study the apparent protection, we looked for sequestered virus in gut tissues, lymph nodes, spleen, and testes obtained at necropsy using virus co-culture and nested PCR for SIV Gag, Pol and LTR. There was no evidence of sequestered virus in tissues obtained from the four protected macaques. In contrast, at least one tissue from each of 11 infected animals scored positive by one of these sensitive procedures. Activated PBMC from the protected macaques were not inherently resistant to in vitro infection by the challenge virus. These findings demonstrate that some vaccinated macaques appeared to be free from the challenge virus. Therefore, such T cell-based vaccines may provide some protection when challenge virus doses approach physiological equivalencies.

Original languageEnglish (US)
Pages (from-to)6474-6481
Number of pages8
JournalVaccine
Volume25
Issue number35
DOIs
StatePublished - Aug 29 2007
Externally publishedYes

Keywords

  • DNA/MVA vaccine
  • GALT
  • Sequestered virus

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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