Abnormal myofiber morphology and limb dysfunction in claudication

Panagiotis Koutakis, Sara A. Myers, Kim Cluff, Duy M. Ha, Gleb Haynatzki, Rodney D. McComb, Koji Uchida, Dimitrios Miserlis, Evlampia Papoutsi, Jason M. Johanning, George P. Casale, Iraklis I. Pipinos

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Background Peripheral artery disease (PAD), which affects an estimated 27 million people in Europe and North America, is caused by atherosclerotic plaques that limit blood flow to the legs. Chronic, repeated ischemia in the lower leg muscles of PAD patients is associated with loss of normal myofiber morphology and myofiber degradation. In this study, we tested the hypothesis that myofiber morphometrics of PAD calf muscle are significantly different from normal calf muscle and correlate with reduced calf muscle strength and walking performance. Methods Gastrocnemius biopsies were collected from 154 PAD patients (Fontaine stage II) and 85 control subjects. Morphometric parameters of gastrocnemius fibers were determined and evaluated for associations with walking distances and calf muscle strength. Results Compared with control myofibers, PAD myofiber cross-sectional area, major and minor axes, equivalent diameter, perimeter, solidity, and density were significantly decreased (P < 0.005), whereas roundness was significantly increased (P < 0.005). Myofiber morphometric parameters correlated with walking distances and calf muscle strength. Multiple regression analyses demonstrated myofiber cross-sectional area, roundness, and solidity as the best predictors of calf muscle strength and 6-min walking distance, whereas cross-sectional area was the main predictor of maximum walking distance. Conclusions Myofiber morphometrics of PAD gastrocnemius differ significantly from those of control muscle and predict calf muscle strength and walking distances of the PAD patients. Morphometric parameters of gastrocnemius myofibers may serve as objective criteria for diagnosis, staging, and treatment of PAD.

Original languageEnglish (US)
Pages (from-to)172-179
Number of pages8
JournalJournal of Surgical Research
Volume196
Issue number1
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

Keywords

  • Claudication
  • Myofiber morphology
  • Walking distance

ASJC Scopus subject areas

  • Surgery

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    Koutakis, P., Myers, S. A., Cluff, K., Ha, D. M., Haynatzki, G., McComb, R. D., Uchida, K., Miserlis, D., Papoutsi, E., Johanning, J. M., Casale, G. P., & Pipinos, I. I. (2015). Abnormal myofiber morphology and limb dysfunction in claudication. Journal of Surgical Research, 196(1), 172-179. https://doi.org/10.1016/j.jss.2015.02.011