Aberrant epigenetic modifications in radiation-resistant head and neck cancers

Hon Yi Lin, Tim Hui Ming Huang, Michael Wing Yan Chan

Research output: Contribution to journalArticlepeer-review

Abstract

Head and neck cancers are characterized by both genetic and epigenetic aberrations. In treating head and neck cancers, ionizing radiation (IR) is an essential modality in either definitive or adjuvant setting. However, radiation-resistant head and neck cancers are not uncommon. The major biological determinator for IR resistance was previously considered at genetic level because DNA is the major target of irradiation damage. However, in head and neck cancers, recent evidence demonstrated epigenetic disturbance after IR, implicating its role in IR resistance. Hence, this chapter intends to establish an in vitro model for investigating DNA methylation changes in IR-resistant head and neck cancer cells. Bisulfite pyrosequencing is the main methodology it introduced.

Original languageEnglish (US)
Pages (from-to)321-332
Number of pages12
JournalMethods in Molecular Biology
Volume1238
DOIs
StatePublished - Jan 1 2015

Keywords

  • DNA methylation
  • Epigenetics
  • Head and neck cancers
  • Histone modification
  • Ionizing radiation
  • Radioresistance
  • Radiosensitivity
  • microRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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