@article{ed0c7154448f4115aafe16df72382412,
title = "A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions",
abstract = "DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes inrecognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing aparticular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects ofSaw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.",
author = "Prabha Sarangi and Veronika Altmannova and Cory Holland and Zdenka Bartosova and Fanfan Hao and Dorothea Anrather and Gustav Ammerer and Lee, {Sang Eun} and Lumir Krejci and Xiaolan Zhao",
note = "Funding Information: We thank Steve Brill for the Slx4 expression plasmid. We are grateful to X.Z. lab members for discussions and useful suggestions. This work is supported by NIH grant GM071011 to S.E.L.; Czech Science Foundation (GACR 13-26629S and 207/12/2323), European Regional Development Fund (Project FNUSA-ICRC; no. CZ.1.05/1.1.00/02.0123) and CZ.1.07/2.3.00/20.0011 cofinanced by European Social Fund and the state budget of the Czech Republic to L.K.; “Employment of Newly Graduated Doctors of Science for Scientific Excellence” (CZ.1.07/2.3.00/30.0009) cofinanced by European Social Fund to V.A.; NIH grant GM080670, American Cancer Society grant RSG-12-013-01-CCG, and Leukemia and Lymphoma Society Scholar Award to X.Z. Funding for open access charge was provided by the NIH. Publisher Copyright: {\textcopyright} 2014 The Authors.",
year = "2014",
month = oct,
day = "9",
doi = "10.1016/j.celrep.2014.08.054",
language = "English (US)",
volume = "9",
pages = "143--152",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "1",
}