A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions

Prabha Sarangi, Veronika Altmannova, Cory Holland, Zdenka Bartosova, Fanfan Hao, Dorothea Anrather, Gustav Ammerer, Sang Eun Lee, Lumir Krejci, Xiaolan Zhao

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes inrecognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing aparticular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects ofSaw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.

Original languageEnglish (US)
Pages (from-to)143-152
Number of pages10
JournalCell Reports
Volume9
Issue number1
DOIs
StatePublished - Oct 9 2014

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Sumoylation
Scaffolds
Repair
DNA Repair
DNA
Proteins
Saccharomycetales
Deoxyribonucleases
DNA Adducts
Yeast
Enzymes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sarangi, P., Altmannova, V., Holland, C., Bartosova, Z., Hao, F., Anrather, D., ... Zhao, X. (2014). A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions. Cell Reports, 9(1), 143-152. https://doi.org/10.1016/j.celrep.2014.08.054

A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions. / Sarangi, Prabha; Altmannova, Veronika; Holland, Cory; Bartosova, Zdenka; Hao, Fanfan; Anrather, Dorothea; Ammerer, Gustav; Lee, Sang Eun; Krejci, Lumir; Zhao, Xiaolan.

In: Cell Reports, Vol. 9, No. 1, 09.10.2014, p. 143-152.

Research output: Contribution to journalArticle

Sarangi, P, Altmannova, V, Holland, C, Bartosova, Z, Hao, F, Anrather, D, Ammerer, G, Lee, SE, Krejci, L & Zhao, X 2014, 'A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions', Cell Reports, vol. 9, no. 1, pp. 143-152. https://doi.org/10.1016/j.celrep.2014.08.054
Sarangi P, Altmannova V, Holland C, Bartosova Z, Hao F, Anrather D et al. A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions. Cell Reports. 2014 Oct 9;9(1):143-152. https://doi.org/10.1016/j.celrep.2014.08.054
Sarangi, Prabha ; Altmannova, Veronika ; Holland, Cory ; Bartosova, Zdenka ; Hao, Fanfan ; Anrather, Dorothea ; Ammerer, Gustav ; Lee, Sang Eun ; Krejci, Lumir ; Zhao, Xiaolan. / A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions. In: Cell Reports. 2014 ; Vol. 9, No. 1. pp. 143-152.
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