Abstract
DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes inrecognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing aparticular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects ofSaw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 143-152 |
| Number of pages | 10 |
| Journal | Cell Reports |
| Volume | 9 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 9 2014 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions. / Sarangi, Prabha; Altmannova, Veronika; Holland, Cory; Bartosova, Zdenka; Hao, Fanfan; Anrather, Dorothea; Ammerer, Gustav; Lee, Sang Eun; Krejci, Lumir; Zhao, Xiaolan.
In: Cell Reports, Vol. 9, No. 1, 09.10.2014, p. 143-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A versatile scaffold contributes to damage survival via sumoylation and nuclease interactions
AU - Sarangi, Prabha
AU - Altmannova, Veronika
AU - Holland, Cory
AU - Bartosova, Zdenka
AU - Hao, Fanfan
AU - Anrather, Dorothea
AU - Ammerer, Gustav
AU - Lee, Sang Eun
AU - Krejci, Lumir
AU - Zhao, Xiaolan
PY - 2014/10/9
Y1 - 2014/10/9
N2 - DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes inrecognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing aparticular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects ofSaw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
AB - DNA repair scaffolds mediate specific DNA and protein interactions in order to assist repair enzymes inrecognizing and removing damaged sequences. Many scaffold proteins are dedicated to repairing aparticular type of lesion. Here, we show that the budding yeast Saw1 scaffold is more versatile. It helps cells cope with base lesions and protein-DNA adducts through its known function of recruiting the Rad1-Rad10 nuclease to DNA. In addition, it promotes UV survival via a mechanism mediated by its sumoylation. Saw1 sumoylation favors its interaction with another nuclease Slx1-Slx4, and this SUMO-mediated role is genetically separable from two main UV lesion repair processes. These effects ofSaw1 and its sumoylation suggest that Saw1 is a multifunctional scaffold that can facilitate diverse types of DNA repair through its modification and nuclease interactions.
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UR - http://www.scopus.com/inward/citedby.url?scp=84907965952&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2014.08.054
DO - 10.1016/j.celrep.2014.08.054
M3 - Article
C2 - 25263559
AN - SCOPUS:84907965952
VL - 9
SP - 143
EP - 152
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 1
ER -