A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex

Kerry O'Donnell, Cécile Gueidan, Stacy Sink, Peter R. Johnston, Pedro W. Crous, Anthony Glenn, Ron Riley, Nicholas C. Zitomer, Patrick Colyer, Cees Waalwijk, Theo van der Lee, Antonio Moretti, Seogchan Kang, Hye Seon Kim, David M. Geiser, Jean H. Juba, Robert P. Baayen, Matthew G. Cromey, Sean Bithell, Deanna A. SuttonKerstin Skovgaard, Randy Ploetz, H. Corby Kistler, Monica Elliott, Mike Davis, Brice A.J. Sarver

Research output: Contribution to journalArticle

180 Scopus citations

Abstract

We constructed a two-locus database, comprising partial translation elongation factor (EF-1α) gene sequences and nearly full-length sequences of the nuclear ribosomal intergenic spacer region (IGS rDNA) for 850 isolates spanning the phylogenetic breadth of the Fusarium oxysporum species complex (FOSC). Of the 850 isolates typed, 101 EF-1α, 203 IGS rDNA, and 256 two-locus sequence types (STs) were differentiated. Analysis of the combined dataset suggests that two-thirds of the STs might be associated with a single host plant. This analysis also revealed that the 26 STs associated with human mycoses were genetically diverse, including several which appear to be nosocomial in origin. A congruence analysis, comparing partial EF-1α and IGS rDNA bootstrap consensus, identified a significant number of conflicting relationships dispersed throughout the bipartitions, suggesting that some of the IGS rDNA sequences may be non-orthologous. We also evaluated enniatin, fumonisin and moniliformin mycotoxin production in vitro within a phylogenetic framework.

Original languageEnglish (US)
Pages (from-to)936-948
Number of pages13
JournalFungal Genetics and Biology
Volume46
Issue number12
DOIs
StatePublished - Dec 2009

Keywords

  • Beauvericin
  • EF-1α
  • Enniatin
  • Fumonisin
  • Fusariosis
  • Genealogical discordance
  • IGS rDNA
  • Moniliformin
  • Mycotoxin
  • Phylogeny
  • Vascular wilt

ASJC Scopus subject areas

  • Microbiology
  • Genetics

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  • Cite this

    O'Donnell, K., Gueidan, C., Sink, S., Johnston, P. R., Crous, P. W., Glenn, A., Riley, R., Zitomer, N. C., Colyer, P., Waalwijk, C., Lee, T. V. D., Moretti, A., Kang, S., Kim, H. S., Geiser, D. M., Juba, J. H., Baayen, R. P., Cromey, M. G., Bithell, S., ... Sarver, B. A. J. (2009). A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genetics and Biology, 46(12), 936-948. https://doi.org/10.1016/j.fgb.2009.08.006